LASER INDUCED THERMOSPRAY IONIZATION FOR PM SPECTROMETRY

质谱新电离技术的发展 以及新的和改进的质谱仪设计的发展 在过去的十年里， 通过质谱技术分析的材料。 生物相关 材料尤其受益于这些进步。 脉冲质量 光谱仪对于生物学相关的 材料，因为它们表现出的高质量范围。 一个潜在 一般来说，脉冲质谱仪的有用电离方法， TOF-MS特别是热喷雾技术。 由于 电离步骤的连续性质，目前不是 作为脉冲离子源实现。 在此建议开发一个 热喷雾电离的变体，其将与脉冲电离兼容， 质谱仪 这将通过执行离子在 如在传统的热喷涂技术中所做的那样。 相反 在直接引入离子源的情况下，流出物（穆尔/分钟流量 速率）将作为气溶胶喷射到旋转的金属鼓上， 连接到可变温度低温冷头上。 流出物 会被冻成一条类似理发师的窄带， 收集表面的螺旋运动。 此后， 流出物将被旋转到高功率脉冲激光器的聚焦光束中， 二氧化碳激光器，将解吸废液和溶剂在脉冲 方式 在合适的实验条件下， 预期离子将以类似于热喷射的方式形成 当膨胀的溶剂团簇蒸发并喷射出完整的分子时 离子。 一个预期的附加优势，提出的电离技术 它与微量和毛细管HPLC方法的兼容性， 在样本有限的情况下， 对于毛细管HPLC，混合物的分辨能力更高。

The development of new ionization techniques for mass spectrometry together with developments in new and improved mass spectrometer designs has in the last decade allowed increasing large and non-volatile materials to be analyzed by mass spectrometric techniques. Bio-relevant materials have benefited especially from these advances. Pulsed mass spectrometers are particularly attractive for biologically relevant materials due to the high mass range which they exhibit. A potentially useful ionization method for pulsed mass spectrometers in general and TOF-MS in particular is the thermospray technique. Due to the continuous nature of the ionization step, it is not currently implemented as a pulsed ion source. It is proposed here to develop a variant of thermospray ionization which will be compatible with pulsed mass spectrometers. This will be accomplished by performing ions in solution as is done in the conventional thermospray technique. Instead of direct introduction into the ion source, the effluent (muL/min. flow rates) will be sprayed as an aerosol onto a rotating metal drum which is attached to a variable temperature cryogenic coldhead. The effluent will be frozen out as a narrow band resembling a barber pole due to the corkscrew motion of the collecting surface. Subsequent to this, the effluent will be rotated into the focused beam of a high powered pulsed carbon dioxide laser which will desorb effluent and solvent in a pulsed manner. Under suitable experimental conditions to be determined, it is expected that ions will be formed in an analogous manner to thermospray as the expanding solvent clusters evaporate and eject intact molecular ions. An expected added advantage to the proposed ionization technique will be its compatibility with micro and capillary HPLC methods which should exhibit increased sensitivity for sample limited cases and in the case of capillary HPLC, higher resolving power for mixtures.