ANALYZE GENE SMR3 CONFERRING MULTIPLE DRUG RESISTANCE

本提案中所述的实验旨在 分析了一个新克隆的基因SMR3。 自发变化. 该基因座赋予酵母多重抗药性。 遗传 对该位点的分析对于几个 原因 首先，这样的研究可以帮助我们理解这一现象 多药耐药的临床意义，但遗传 例如念珠菌、疟原虫和人类。 其次，酵母基因座在结构上很有趣。 突变 抗药性是不稳定的。 此外，SMR3似乎是 一个复杂的多位点区域的成员，其介导药物 阻力 通过使用经典遗传学，核酸 杂交技术和DNA测序，我们应该学习 这种基因是否与肿瘤对应物相似， 观察到的不稳定性的基础。

The experiments described in this proposal are aimed at the analysis of a newly cloned gene, SMR3. Spontaneous alterations at this locus confer multiple drug resistance in yeast. Genetic analysis of this locus is particularly important for several reasons. First, such a study may help us understand the phenomenon of multidrug resistance in clinically significant, but genetically cumbersome organisms such as Candida, Plasmodium and humans. Secondly, the yeast locus is structurally intriguing. Mutations of drug resistance are unstable. Furthermore, SMR3 appears to be a member of a complex multi-locus region that mediates drug resistance. By using classical genetics, nucleic acid hybridization techniques and DNA sequencing, we should learn whether this gene is analogous to the tumor counterpart as well as the basis of the observed instability.