ANALYZE GENE SMR3 CONFERRING MULTIPLE DRUG RESISTANCE

分析赋予多重耐药性的基因 SMR3

• 批准号: 3438707
• 负责人: JOHN E GOLIN
• 金额: $ 10.45万
• 依托单位: CATHOLIC UNIVERSITY OF AMERICA
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3438707
• 关键词: endonuclease; fungal genetics; gene expression; gene mutation; genetic manipulation; genetic mapping; molecular cloning; molecular genetics; natural gene amplification; nucleic acid hybridization; nucleic acid sequence

The experiments described in this proposal are aimed at the analysis of a newly cloned gene, SMR3. Spontaneous alterations at this locus confer multiple drug resistance in yeast. Genetic analysis of this locus is particularly important for several reasons. First, such a study may help us understand the phenomenon of multidrug resistance in clinically significant, but genetically cumbersome organisms such as Candida, Plasmodium and humans. Secondly, the yeast locus is structurally intriguing. Mutations of drug resistance are unstable. Furthermore, SMR3 appears to be a member of a complex multi-locus region that mediates drug resistance. By using classical genetics, nucleic acid hybridization techniques and DNA sequencing, we should learn whether this gene is analogous to the tumor counterpart as well as the basis of the observed instability.

本提案中所描述的实验是针对

项目成果

Mutations in the yeast PDR3, PDR4, PDR7 and PDR9 pleiotropic (multiple) drug resistance loci affect the transcript level of an ATP binding cassette transporter encoding gene, PDR5.

酵母 PDR3、PDR4、PDR7 和 PDR9 多效性（多个）耐药位点的突变会影响 ATP 结合盒转运蛋白编码基因 PDR5 的转录水平。

• DOI: 10.1093/genetics/136.2.505
• 发表时间: 1994
• 期刊: Genetics
• 影响因子: 3.3
• 作者: Dexter,D;Moye-Rowley,WS;Wu,AL;Golin,J
• 通讯作者: Golin,J

Cloning by gene amplification of two loci conferring multiple drug resistance in Saccharomyces.

通过基因扩增克隆酵母菌中赋予多重耐药性的两个基因座。

• DOI: 10.1093/genetics/125.1.13
• 发表时间: 1990
• 期刊: Genetics
• 影响因子: 3.3
• 作者: Leppert,G;McDevitt,R;Falco,SC;VanDyk,TK;Ficke,MB;Golin,J
• 通讯作者: Golin,J