Analysis of Role of the Yeast UBP6-encoded Hydrolase

描述(申请人提供)：在酵母中，广谱耐药 抑制剂是由一系列膜转运蛋白介导的，这些膜转运蛋白是 ABC超家族并使用三磷酸腺苷为药物外排提供动力。涉及的基因 包括PDR5、YOR1和SNQ2。这些基因座又受锌簇的调控。 由PDR1、YRR1(PDR2)和PDR3基因编码的转录因子。 PDR2的显性功能增益突变通过Over YOR1、SNQ2和至少一个其他靶标的表达。奇怪的是，这个目标 需要Ubp6泛素水解酶的存在。UBP6基因零突变 对蛋白质翻译的抑制剂产生过敏反应。使用微阵列 分析后，将识别依赖于Ubp6的PDR2目标。后续遗传 分析将确定与UBP6一起工作的其他基因。这部作品，沿着 一些简单的分子细胞学应该有助于确定 细胞中的这种水解酶。

DESCRIPTION (provided by applicant): In yeast, broad-spectrum resistance inhibitors is mediated by a series of membrane transporters that are members of the ABC superfamily and use ATP to power drug efflux. The genes involved include PDR5, YOR1, and SNQ2. These loci are in turn regulated by zinc cluster transcriptionfactors encoded by the PDR1, YRR1 (PDR2) and PDR3 genes. Dominant gain-of-function mutations in PDR2 cause hyper-resistance via the over expression of YOR1, SNQ2 and at least one other target. Curiously, this target requires the presence of the Ubp6 ubiquitin hydrolase. Null mutations in UBP6 create hypersensitivity to inhibitors of protein translation. Using microarray analysis, the Ubp6-dependent PDR2 target will be identified. Subsequent genetic analysis will identify other genes that work along with UBP6. This work, along with some simple molecular cytology should help determine the precise role of this hydrolase in the cell.