DETECTION METHODS FOR DNA-DAMAGING AGENTS IN MAN

人类 DNA 损伤剂的检测方法

• 批准号: 3433938
• 负责人: HELMUT BARTSCH
• 金额: $ 2万
• 依托单位: INTERNATIONAL AGENCY FOR RES ON CANCER
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-06-01 至 1988-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3433938
• 关键词: carcinogen testing; chemical carcinogen; mutagen testing; travel

A multidisciplinary conference at IARC (September 2-4, 1987, Lyon France) will convene about 100 scientists to present and discuss the effective application of sensitive methods for monitoring exposure to DNA-damaging agents by biochemical epidemiology. The meeting will focus on chemical and biochemical dosimetry methods which allow monitoring of human exposure to genotoxic chemicals and mixtures which are of significance for cancer induction in man: mycotoxins, polycyclic aromatic hydrocarbons, nitrosamines, aromatic amines, epoxides, anti-cancer drugs, endogenously formed genotoxic substances, as well as number of complex mixtures. Methods include the detection in body fluids and tissues of carcinogens/metabolites and their DNA, RNA and protein adducts by physical, chemical and immunological techniques, such as immuno assays, fluorsecence and scanning fluorimetry, GC-MS, 32P-postlabelling; analysis of protein adducts, urine analyses for modified nucleic acid bases and indicator nitrosamines; body fluid analysis for mutagens. The meeting will evaluate, on an international level, the significance, the current stage of development and potential use of these dosimetry methods, and give a critical appraisal of proper study design, when applied in human studies. Current applications of these methods in human studies will be reviewed with emphasis on: a) identification of carcinogenic hazards and unknown exposures, b) identification of high-risk groups/subjects, and c) an assessment of the efficiency of cancer- protective agents and intervention trials in reducing carcinogen exposure. The conference and the proceedings volume will give guidance for the best co-ordinated research efforts and worldwide priorities for conducting studies in molecular epidemiology of carcinogens.

IARC举行的多学科会议（1987年9月2日至4日， 法国里昂）将召集大约100位科学家，并 讨论敏感方法的有效应用 通过生化监测对DNA损害剂的暴露 流行病学。 会议将重点放在化学和生化剂量法上 允许监测人类对遗传毒性暴露的方法 对癌症具有重要意义的化学和混合物 人类的诱导：霉菌毒素，多环芳烃， 亚硝胺，芳香胺，环氧化物，抗癌药物， 内源形成的遗传毒性物质以及数量 复杂的混合物。 方法包括在体液和组织中的检测 致癌物/代谢产物及其DNA，RNA和蛋白质加合物 通过物理，化学和免疫学技术，例如 免疫测定，荧光和扫描荧光法，GC-MS， 32p postlabelling;分析蛋白质加合物，尿液分析 修饰的核酸碱和指示剂亚硝基胺；体液 分析诱变剂。 会议将在国际层面评估 意义，当前的发展阶段和潜在用途 这些剂量法，并对 适当的研究设计，当应用于人类研究中时。 这些方法在人类研究中的当前应用将是 重点审查：a）识别致癌 危害和未知暴露，b）识别高风险 组/受试者，c）评估癌症的效率 减少致癌的保护剂和干预试验 接触。 会议和诉讼程序将为 最好的协调研究工作和全球优先事项 用于进行致癌物分子流行病学的研究。