CHARACTERIZATION OF A CELLOBIOSE UTILIZING E. COLI

利用大肠杆菌表征纤维二糖

• 批准号: 3436638
• 负责人: MICHAEL H SCHOLLA
• 金额: $ 7.11万
• 依托单位: UNIVERSITY OF MEMPHIS
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-30 至 1988-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3436638
• 关键词: Enterobacteriaceae; Escherichia coli; Escherichia coli k12; affinity chromatography; autoradiography; bacterial genetics; bacteriophage lambda; beta glucosidases; electrophoresis; gene expression; genetic manipulation; genetic mapping; genetic recombination; microorganism metabolism; molecular cloning; mutant; nucleic acid sequence; radiotracer; transposon /insertion element

This proposal involves the characterization of a cellobiose utilizing mutant from E. coli B. Characterization of the mutant will be accomplished using biochemical and genetic approaches. Our goals include the elucidation and characterization of the specific proteins required for transport and catabolism of cellobiose. We also propose to determine the number, location and function of genes required for cellobiose utilization. Preliminary characterization of the cellobiose+ E. coli strain indicates the mechanism for cellobiose transport and metabolism is different from previously described systems. This study has the potential for providing new information regarding the catabolism of cellobiose by bacteria.

该提议涉及利用以下方法表征纤维二糖： 突变株E.大肠杆菌B。 将完成突变体的表征 使用生物化学和遗传学方法。 我们的目标包括 所需的特定蛋白质的阐明和表征 纤维二糖的运输和催化。 我们还建议确定 纤维二糖所需基因的数量、位置和功能 利用率 纤维二糖+ E.杆菌 菌株表明，纤维二糖的运输和代谢机制是 不同于先前描述的系统。 这项研究有可能 提供了关于纤维二糖催化剂的新信息， 细菌