THREE-DIMENSIONAL ELECTRON MICROSCOPY OF MACROMOLECULES

拟议中的会议将是戈登新系列会议的第五次 大分子三维电子显微镜会议。 电子显微镜作为一门功能强大的学科，近年来发展迅速。 在分子水平上研究结构的工具。重要技术 已经取得了突破，使三维结构能够 以越来越准确的方式确定，哪些已经打开了通往 结构本质上受控生理的研究 条件。因此，出现了令人振奋的新机会 了解分子设计的生物学机制和原理。 这一系列会议的独特地位源于这样一个事实 它们有助于培养对生物复杂性的综合看法，通过 将致力于不同层次的科学家聚集在一起 结构层次。这次会议的重点将是联合起来 关于生物学的准备和可视化的几点想法 横跨非常宽的大小范围的样本，从 从近原子一直到细胞，甚至更远。最近的重要事件 结构发现，尤其与膜蛋白、病毒、 细胞细胞器和细胞骨架结构将在 除了仪器和计算的实际方面 三维重建的方法。 这次会议将有助于促进该领域的进一步发展。 通过将许多关键调查人员聚集在一起并允许他们 讨论他们的最新研究，甚至可能是不完整的研究 独特而非正式的环境。演讲者是根据以下因素选择的 他们最近研究贡献的重要性和潜力 他们在未来结构研究中的特殊方法。待讨论的主题 内容包括：混合技术(电子和x射线工作 一起)，在结晶、计算和仪器方面的进展， 特定部位的非低温制备技术的重生 大分子标记，三维重建方法， 图像形成、获取和显示的进展及最新进展 获得了各种大分子和细胞的结构结果 程序集和病毒。

The proposed conference will be the fifth of a new series of Gordon Conferences on Three-dimensional Electron Microscopy of Macromolecules. Electron microscopy has developed rapidly in recent years as a powerful tool for structure research at the molecular level. Important technical breakthroughs have been made which enable three-dimensional structure to be determined with increasing accuracy, and which have opened the way to a study of structure essentially under controlled physiological conditions. As a result, there are exciting new opportunities for understanding biological mechanisms and principles of molecular design. The unique position of this series of conferences derives from the fact that they help foster an integrated view of biological complexity, by bringing together scientists working on different levels of the structural hierarchy. The focus of this meeting will be to coalesce ideas concerning the preparation and visualization of biological specimens that span an extremely wide size range, extending from the near-atomic all the way to the cellular and beyond. Important recent structural findings, relating especially to membrane proteins, viruses, cell organelles, and cytoskeletal structures will be emphasized in addition to practical aspects of instrumentation and computational methods of three-dimensional reconstruction. This conference will help catalyze the further development of this field by bringing together many of the key investigators and allowing them to discuss their latest studies and perhaps even incomplete studies in a unique and informal setting. Speakers are being chosen on the basis of the importance of their recent research contributions and the potential of their particular approach in future structure research. Topics to be covered include: hybrid technology (electrons and x-rays working together), advances in crystallization, computation, and instrumentation, a rebirth of non-cryogenic preparation techniques, site-specific labelling of macromolecules, methods of three-dimensional reconstruction, progress on image formation, acquisition, and display, and recent structural results obtained for a variety of macromolecular and cellular assemblies and viruses.