IMMUNOPROTECTIVE POTENTIAL OF PORINS AND ANTI-PORIN MABS

描述（改编自申请人摘要）：本提案涉及 鼠伤寒沙门氏菌免疫保护作用的研究 孔蛋白和抗孔蛋白单克隆抗体 沙门氏菌病 将用一组7只小鼠免疫各组小鼠。 抗OmpD（三聚体）和11种抗OmpC（三聚体）单克隆抗体， 已经显示与暴露的表位结合。 第二组小鼠将 用天然三聚体形式的沙门氏菌孔蛋白免疫。 这些 小鼠将用2至100 LD 50值的毒性S. 鼠伤寒杆菌LT-2。 孔蛋白和抗孔蛋白的保护功效 单克隆抗体将从21天存活数据确定。 一 第三组小鼠将口服致死剂量的沙门氏菌 伴随腹水，在给药前给予激发剂量或IP 感染剂量。 派伊尔集结地沙门氏菌的数量 将在给药后间隔测定，以使 口服给予抗孔蛋白单克隆抗体以阻断 沙门氏菌的入侵

DESCRIPTION (Adapted from applicant's abstract): This proposal deals with the study of the immunoprotective potential of Salmonella typhimurium porins and anti-porin monoclonal antibodies in experimental murine salmonellosis. Groups of mice will be immunized with a panel of 7 anti-OmpD (trimer) and 11 anti-OmpC (trimer) monoclonal antibodies that have been shown to bind to exposed epitopes. A second group of mice will be immunized with Salmonella porins in their native trimeric forms. These mice will be challenged with 2 to 100 LD50 values of virulent S. typhimurium LT-2. The protective efficacy of porins and anti-porin monoclonal antibodies will be determined from 21-day survival data. A third group of mice will be given, orally, a lethal dose of Salmonella accompanied by ascites fluid either with the challenge dose or IP prior to the infectious dose. The number of Salmonella resident in Peyer's patches will be determined at intervals after administration in order to correlate the efficacy of anti-porin monoclonal antibodies given orally to block the invasion of Salmonella.