IMMUNOPROTECTIVE POTENTIAL OF PORINS AND ANTI-PORIN MABS

描述(改编自申请人的摘要)：本提案涉及 鼠伤寒沙门氏菌免疫保护作用的研究 实验性小鼠的孔蛋白及抗孔蛋白单抗 沙门氏菌病。一组小鼠将用一个7人小组进行免疫 抗OmpD(三聚体)和11抗OMPC(三聚体)单抗 已经被证明可以与暴露的表位结合。第二组小鼠将 以天然三聚体形式的沙门氏菌孔蛋白进行免疫。这些 小鼠将受到2到100个强毒S的LD50值的挑战。 小鼠伤寒杆菌LT-2。毛孔蛋白和抗毛孔蛋白的保护作用 单抗将从21天的存活数据中确定。一个 第三组小鼠将口服致死剂量的沙门氏菌 伴有腹水，或有激发剂量或ip前 感染性剂量。Peyer‘s斑块中沙门氏菌的数量 将在给药后每隔一段时间确定，以便相互关联 口服抗孔蛋白单抗阻断血管内皮细胞瘤的疗效 沙门氏菌入侵。

DESCRIPTION (Adapted from applicant's abstract): This proposal deals with the study of the immunoprotective potential of Salmonella typhimurium porins and anti-porin monoclonal antibodies in experimental murine salmonellosis. Groups of mice will be immunized with a panel of 7 anti-OmpD (trimer) and 11 anti-OmpC (trimer) monoclonal antibodies that have been shown to bind to exposed epitopes. A second group of mice will be immunized with Salmonella porins in their native trimeric forms. These mice will be challenged with 2 to 100 LD50 values of virulent S. typhimurium LT-2. The protective efficacy of porins and anti-porin monoclonal antibodies will be determined from 21-day survival data. A third group of mice will be given, orally, a lethal dose of Salmonella accompanied by ascites fluid either with the challenge dose or IP prior to the infectious dose. The number of Salmonella resident in Peyer's patches will be determined at intervals after administration in order to correlate the efficacy of anti-porin monoclonal antibodies given orally to block the invasion of Salmonella.