ENRICHMENT ACTIVITIES

拟议的浓缩计划的目标是：(1)提供 少数族裔理科学生有机会结识和 参与生物医学研究，以激励他们追求 生物医学的研究生课程；及(2)提供 为理科教师提供提升研究技能和 积极参与赞助研究和学生培训。这个 拟议的活动包括：(A)教职员工和学生参与 阿拉巴马州立大学来访科学家举办的研讨会和讲习班 大学校园，(B)教职员工参加讲习班，校外 研究，以及校外研究的“重组”体验 机构，(C)学生参与校外生物医学 暑假期间在美国主要大学进行的研究， (D)选修BIO350生物医学研究导论的学生 阿拉巴马州立大学的技术(E)教职员工和学生 参加阿拉巴马州立大学生物医学科学俱乐部 活动与文理学院的年度研究 研讨会，以及(F)出席(教职员工和学生)和演讲 在国家科学会议上发表研究论文。我们相信这些 活动将有助于提高智力和科学水平 自然科学中的环境将激励更多的科学教师 积极参与研究，追求更多的学生 生物医学的研究生课程。 GRANT=S06GM082190006 鼠伤寒沙门氏菌是一种革兰氏阴性病原体，主要引起 人的胃肠炎，但会引起小鼠的伤寒样疾病。 鼠伤寒沙门氏菌和其他革兰氏阴性菌的外膜 细菌含有内毒素和一系列主要蛋白质。 叫色情片。这些OM组件是免疫的主要目标 在细菌感染期间被宿主识别。这个项目将 对编码OmpD孔蛋白的基因进行测序，并使用单抗 (单抗)以确定体液免疫的分子特异性 对鼠伤寒沙门氏菌感染的反应。《公约》的具体目标 项目是绘制沙门氏菌OmpD孔蛋白的抗原区图， 克隆和测序ompD基因，鉴定显性和非显性 免疫保护性孔蛋白和孔蛋白-内毒素表位，并确定 内毒素结构对这些表位识别的影响 细菌感染。OmpD的抗原区将由 溴化氰生孔蛋白的免疫印迹反应 与一组现有的抗OmpD单抗结合的多肽。身份识别 主要的(和免疫保护的)表位将涉及分析 结合竞争多克隆免疫血清(酶联) 免疫吸附试验和Western blotts)与一组现有的抗 沙门氏菌单抗。这些单抗要么是针对表面表位的 OmpD或OMPC孔蛋白，或孔蛋白-内毒素复合体，它们或者 延长小鼠的存活时间或提供显著的保护 实验性小鼠沙门氏菌病的内毒素血症和/或菌血症。这个 内毒素结构对抗孔蛋白和抗内毒素特异性的影响 在感染过程中产生的抗体将使用Smooth和 鼠伤寒沙门氏菌的粗糙变种。这个项目的长期目标是 阐明免疫优势孔蛋白表位的潜在应用 为了开发沙门氏菌特异性诊断探针和疫苗， 以及外源基因的插入和表达。因为所有的奶奶- 阴性细菌可能含有类似的OM蛋白结构， 拟议的研究与革兰氏阴性细菌的发病机制有关 将军。该项目还旨在培训那些 将参与该研究项目的所有阶段。学生们将 查阅文献，制定研究思路，设计并实施 使用现代生物医学研究技术的实验，并获得 在地区学术会议上撰写和发表科学论文的经验 以及全国性的会议。

The objectives of the proposed enrichment plan are: (1) to provide opportunities for minority science students to become acquainted with and to participate in biomedical research in order to motivate them to pursue graduate studies in the biomedical sciences, and (2) to provide opportunities for science faculty to upgrade their research skills and be involved in active sponsored research and student training. The proposed activities include: (a) faculty and student participation in seminars and workshops conducted by visiting scientists at Alabama State University campus, (b) faculty participation in workshops, extramural research, and "retooling" experiences at off-campus research institutions, (c) students' participation in extramural biomedical research during the summer at major universities in the United States, (d) students taking BIO 350, Introduction to Biomedical Research Techniques at Alabama State University, (e) faculty and student participation in Alabama State University's Biomedical Science Club activities and the College of Arts and Science's annual research symposium, and (f) attendance (by faculty and students) and presentation of research papers at national scientific meetings. We believe these activities will contribute to an improved intellectual and scientific environment in natural sciences which will motivate more science faculty to get actively involved in research, and more students to pursue graduate studies in biomedical sciences. Grant=S06GM082190006 Salmonella typhimurium is a gram-negative pathogen that primarily evokes gastroenteritis in man, but causes typhoid fever-like disease in mice. The outer membrane (OM) of S. typhimurium and other gram-negative bacteria contains lipopolysaccharide (LPS) and a family of major proteins called, porins. These OM components are the primary targets for immune recognition by the host during bacterial infections. This project will sequence the gene that encodes OmpD porin, and use monoclonal antibodies (MAbs) to determine the molecular specificity of the humoral immune response to infection by S. typhimurium. The specific aims of the project are to map the antigenic regions on the OmpD porin of Salmonella, to clone and sequence the ompD gene, to identify the dominant and immunoprotective porin and porin-LPS epitopes, and to determine the effect of LPS structure on the recognition of these epitopes during bacterial infections. The antigenic regions of OmpD will be mapped by Western immunoblot reactivity of cyanogen bromide-generated porin peptides with a panel of existing anti-OmpD MAbs. Identification of dominant (and immunoprotective) epitopes will involve the analysis of polyclonal immune sera by binding competition (enzyme-linked immunosorbent assay and Western blots) with a panel of existing anti- Salmonella MAbs. These MAbs are either specific for the surface epitopes of OmpD or OmpC porin, or for the porin-LPS complex, and they either prolong the survival of mice or confer significant protection against endotoxemia and/or bacteremia in experimental murine salmonellosis. The effect of LPS structure on the specificity of anti-porin and anti-LPS antibodies that arise during infection will be studied using smooth and rough variants of S. typhimurium. The long-term goal of this project is to elucidate the potential application of immunodominant porin epitopes for development of Salmonella-specific diagnostic probes and vaccines, and for insertion and expression of foreign genes. Since all gram- negative bacteria probably contain similar OM protein structures, the proposed research is relevant to gram-negative bacterial pathogenesis in general. The project is also designed to train minority students who will participate in all phases of this research project. Students will review literature, formulate research ideas, design and carry out experiments using modern biomedical research techniques, and gain experience in writing and presentation of scientific papers at regional and national meetings.