CYANOPHAGE LYTIC/LYSOGENIC INFECTION OF ANABENA

这个项目的长期目标是阐明 蓝细菌中病毒感染和抗性的机制 应用这些知识，使光合作用的过程， 固氮和细胞分化可以进行更多的研究 有效地。因为这些过程是工厂的基础 对它们的深入了解对未来的发展至关重要 发展农业和改善营养。 特别是蓝藻，可变鱼腥藻将是 独立感染了七种不同的自然发生 病毒(噬藻体)和对每种病毒类型具有抗药性的细胞 与世隔绝。然后将抗病毒细胞类型与 探索病毒感染机制的敏感细胞和 抵抗。这些比较将通过凝胶进行 病毒蛋白的电泳分析，细胞的消化 和病毒DNA的限制性内切酶及其分析 DNA凝胶电泳法和核酸测序法 杂交。DNA片段被鉴定为与 噬藻体感染或抗药性会通过 重组DNA方法在大肠杆菌宿主载体系统中的应用 以获取更详细的研究。

The long term objectives of this project are to elucidate the mechanisms of viral infection and resistance in cyanonbacteria, to apply this knowledge so that the processes of photosynthesis, nitrogen fixation and cellular differentiation can be studied more effectively. Since these processes are fundamental for plant growth thorough understanding of them is crucial to future development of agriculture and improved nutrition. Specifically the cyanobacterium, Anabena variabilis will be infected independently with seven different naturally occurring viruses (cyanophage) and cells resistant to each virus type will be isolated. The virus resistant cell types will then be compared to the sensitive cells to explore the mechanism of virus infection and resistance. These comparisons will be performed by gel electrophoretic analysis of the virus proteins, digestion of cellular and virus DNA with restriction endonucleases and analysis of the DNA fragments by gel electrophoresis and nucleic acid hybridization. DNA fragments identified as involved in cyanophage infection or resistance will be transferred through recombinant DNA methodologies to E. coli host-vector systems for more detailed study.