权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

IN-VITRO TENDON CELL ATTACHMENT TO SYNTHETIC FIBERS

体外肌腱细胞附着于合成纤维

基本信息

批准号：
3446385
负责人：
JOHN RICCI
金额：
$ 5.81万
依托单位：
HOSPITAL FOR JOINT DISEASES ORTHO INST
依托单位国家：
美国
项目类别：
财政年份：
1986
资助国家：
美国
起止时间：
1986-12-01 至 1989-08-31
项目状态：
已结题

项目摘要

Synthetic fiber materials are used in a variety of surgical applications that involve tissue and cell ingrowth and adhesion. The recent use of synthetic fiber-based implants as tissue scaffolds for the regrowth of damaged tendons and ligaments is a notable example. Often these tissues and fiber materials are subjected to mechanical stress. However, little is known concerning the process of cell adhesion to these materials and the effects of mechanical stress on cell adhesion and cell function. The proposed series of experiments is designed to investigate the characteristics of rat tendon fibroblast cell attachment to a variety of synthetic fibers, in vitro, and to investigate the effects of mechanical stress and deformation on these cells. Carbon, Dacron, polyethylene and Nylon fibers of varying diameters, surface characteristics and mechanical properties will be used as substrates. Cell culture techniques, scanning electron microscopy, transmission electron microscopy, immunofluorescent labeling of cell cytoskeleton (anti-actin IgG labeling of stress fibers), reflection interference microscopy and flow viscometry measurement of cell adhesion strength will be used in these experiments. Morphometric methods will be used to evaluate the morphological data. The proposed experiments are designed to investigate: (a) the general morphology, morphology of cell attachment and cell cytoskeleton of rat tendon cells cultured on synthetic fibers of varying compositions and configurations, (b) the strength of rat tendon cell attachment to these fibers, and (c) the effect of mechanical stress and deformation, applied to the fiber substrates, on cell attachment morphology and strength. These experiments will provide valuable information concerning connective tissue cell interaction with a variety of commonly used surgical materials, and information concerning the cellular mechanisms involved with stress-induced remodeling of tissue.

合成纤维材料被用于各种外科手术涉及组织和细胞内生以及粘附力。最近合成纤维为基础的植入物的使用用于损伤肌腱再生的组织支架和韧带就是一个明显的例子。通常这些组织和纤维材料受到机械应力的影响。然而，几乎没有已知的关于细胞与这些细胞黏附的过程材料与机械应力对细胞黏附的影响和细胞功能。拟议的一系列实验旨在研究大鼠肌腱成纤维细胞黏附于多种合成纤维，在体外，并考察了机械应力和变形对这些细胞的影响。各种碳纤维、涤纶纤维、聚乙烯纤维和尼龙纤维直径、表面特征和机械性能将被用作底物。细胞培养技术，扫描电子显微镜，透射电子显微镜，细胞骨架(抗肌动蛋白抗体)的免疫荧光标记应力纤维的标记)，反射干涉显微镜并用流动粘度计测定细胞黏附强度在这些实验中使用。形态测量方法将是用于对形态数据进行评估。建议数实验旨在调查：(A)一般情况下形态、细胞附着形态和细胞骨架大鼠肌腱细胞在不同种类合成纤维上的培养成分和构型，(B)大鼠肌腱的强度细胞附着在这些纤维上，以及(C) 施加到纤维上的机械应力和变形底物对细胞附着形态和强度的影响。这些实验将提供有价值的信息，如结缔组织细胞与多种常见的使用过的手术材料，以及关于应激诱导血管重塑的细胞机制组织。