IN-VITRO TENDON CELL ATTACHMENT TO SYNTHETIC FIBERS

体外肌腱细胞附着于合成纤维

• 批准号: 3446384
• 负责人: JOHN RICCI
• 金额: $ 4.82万
• 依托单位: HOSPITAL FOR JOINT DISEASES ORTHO INST
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-12-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446384
• 关键词: biomaterial evaluation; biomaterial interface interaction; cell adhesion; cytoskeleton; electron microscopy; fibroblasts; flow cytometry; fluorocarbon polymers; histochemistry /cytochemistry; immunofluorescence technique; mechanical stress; particle; plastics; polyethylenes; scanning electron microscopy; tendons; tissue /cell culture; tissue support frame

Synthetic fiber materials are used in a variety of surgical applications that involve tissue and cell ingrowth and adhesion. The recent use of synthetic fiber-based implants as tissue scaffolds for the regrowth of damaged tendons and ligaments is a notable example. Often these tissues and fiber materials are subjected to mechanical stress. However, little is known concerning the process of cell adhesion to these materials and the effects of mechanical stress on cell adhesion and cell function. The proposed series of experiments is designed to investigate the characteristics of rat tendon fibroblast cell attachment to a variety of synthetic fibers, in vitro, and to investigate the effects of mechanical stress and deformation on these cells. Carbon, Dacron, polyethylene and Nylon fibers of varying diameters, surface characteristics and mechanical properties will be used as substrates. Cell culture techniques, scanning electron microscopy, transmission electron microscopy, immunofluorescent labeling of cell cytoskeleton (anti-actin IgG labeling of stress fibers), reflection interference microscopy and flow viscometry measurement of cell adhesion strength will be used in these experiments. Morphometric methods will be used to evaluate the morphological data. The proposed experiments are designed to investigate: (a) the general morphology, morphology of cell attachment and cell cytoskeleton of rat tendon cells cultured on synthetic fibers of varying compositions and configurations, (b) the strength of rat tendon cell attachment to these fibers, and (c) the effect of mechanical stress and deformation, applied to the fiber substrates, on cell attachment morphology and strength. These experiments will provide valuable information concerning connective tissue cell interaction with a variety of commonly used surgical materials, and information concerning the cellular mechanisms involved with stress-induced remodeling of tissue.

合成纤维材料用于各种外科手术 涉及组织和细胞向内生长的应用以及 附着力。 最近使用基于合成纤维的植入物 用于受损肌腱再生的组织支架 韧带就是一个​​显着的例子。 通常这些组织和纤维 材料受到机械应力。 然而，很少 已知关于细胞粘附到这些的过程 材料和机械应力对细胞粘附的影响 和细胞功能。 拟议的一系列实验旨在研究 大鼠肌腱成纤维细胞附着特性 各种合成纤维，在体外，并研究 机械应力和变形对这些细胞的影响。 各种碳纤维、涤纶纤维、聚乙烯纤维和尼龙纤维 直径、表面特性和机械性能 将被用作基材。 细胞培养技术、扫描 电子显微镜、透射电子显微镜、 细胞骨架的免疫荧光标记（抗肌动蛋白 IgG 应力纤维的标记），反射干涉显微镜 和流式粘度计测量细胞粘附强度将 用于这些实验。 形态测量方法将 用于评估形态数据。 拟议的 实验旨在研究：（a）一般 形态学、细胞附着形态和细胞骨架 在不同合成纤维上培养的大鼠肌腱细胞 成分和构造，(b) 大鼠肌腱的强度 细胞附着在这些纤维上，以及（c） 施加到纤维上的机械应力和变形 基质对细胞附着形态和强度的影响。 这些实验将提供有关以下方面的宝贵信息： 结缔组织细胞与多种常见的相互作用 使用的手术材料以及有关的信息 与应激诱导重塑相关的细胞机制 组织。