CHARACTERIZATION OF ACUTE MYELOGENOUS LEUKEMIA STEM CELL
CHARACTERIZATION OF ACUTE MYELOGENOUS LEUKEMIA STEM CELL
批准号:
3446987
负责人:
ALEXANDRA L HOWELL
金额:
$5.26万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-07-01 至 1990-03-31
关键词:
1,25 dihydroxycholecalciferol 13 cis retinoate cell differentiation cell growth regulation cell population study cellular oncology clone cells complement fixation tests gene expression human subject immunofluorescence technique immunohematology interferons leukopoiesis leukopoietic factor molecular oncology monoclonal antibody monocyte morphology myelogenous leukemia myeloid stem cell neutrophil oncogenes surface antigens tissue /cell culture
中文摘要
该项目涉及白血病集落形成细胞的研究。
急性髓系白血病患者的群体(L-氟氯化碳)。在……里面
本研究的一个方面,L-CFCs表达的细胞表面抗原
将在急性髓系白血病患者中使用一组髓系特异性的
单抗和补体介导的细胞毒作用。患者会
在随后的复发时进行连续检查,以确定L-氟氯化碳
表型随时间变化稳定。这些研究的结果将在那时
与一系列旨在检验诱导性的实验相比较
用多种已知作用于髓系的药物促进L-CFCs的成熟
细胞分化。接受检测的试剂包括重组伽马
干扰素、1,25二羟基维生素D3和顺式维甲酸。中的更改
将检查表面抗原、增殖能力和形态。
L-氯氟化碳暴露在最佳浓度下的成熟诱导作用
探员们。这些研究旨在检查急性髓细胞白血病中的L-氟氯化碳是否
对分化因子作出反应,并确定是否有反应
表现为与正常骨髓生成相似的细胞变化
(即,更分化的细胞表型的表达和减少
在细胞增殖中)。
另一个方面是将两者的细胞表面抗原性展示相关联
有癌基因表达的正常和白血病髓系细胞。表达方式
将研究三种髓系相关细胞癌基因(c-onc):
N-ras、c-myc和c-fos。癌基因表达将通过以下方式进行量化
从正常髓系提取的c-onc RNA的探针杂交
亚群(单核细胞和中性粒细胞)和白血病原始细胞
来自急性髓系白血病患者的人群。癌基因蛋白的表达
产品将通过使用抗血清进行免疫荧光研究来确定
癌基因蛋白,并通过免疫沉淀研究放射性标记的
细胞裂解物。这些研究的目的是关联基因的表达
正常和非癌组织中髓系细胞表面抗原模式的癌基因
判断恶性细胞是否与
差异化状态。结果将加深我们对白血病的理解
AML中的细胞生物学,并旨在阐明成熟的
髓系白血病中出现的缺陷。通过确定
L-氟氯化碳人口的成熟能力、替代办法
治疗,包括免疫治疗和成熟诱导治疗,可以
结果。
英文摘要
This project involves the study of the leukemia-colony forming cell
population (L-CFC) in patients with acute myelogenous leukemia (AML). In
one aspect of this study, the cell surface antigens expressed by the L-CFC
will be examined in patients with AML using a panel of myeloid-specific
monoclonal antibodies and complement-mediated cytotoxicity. Patients will
be examined serially on subsequent relapses to determine whether the L-CFC
phenotype is stable with time. The findings from these studies will then
be compared to a series of experiments designed to examine the inducibility
to maturation of the L-CFC using various agents known to effect myeloid
cell differentiation. The agents to be tested include recombinant gamma
interferon, 1,25 dihydroxyvitamin D3, and cis-retinoic acid. Changes in
surface antigens, proliferative potential, and morphology will be examined
in the L-CFC exposed to optimal concentrations of the maturation-inducing
agents. The studies are designed to examine whether the L-CFC in AML is
responsive to agents of differentiation, and to determine whether response
is manifested in cellular changes similar to those of normal myelopoiesis
(i.e., expression of a more differentiated cell phenotype and a reduction
in cellular proliferation).
Another aspect is to correlate the cell surface antigenic display of both
normal and leukemic myeloid cells with oncogene expression. The expression
of three myeloid-associated cellular oncogenes (c-onc) will be studied:
N-ras, c-myc and c-fos. Oncogene expression will be quantified by
hybridization of probes to c-onc RNA extracted from normal myeloid
subpopulations (monocytes and neutrophils) and from leukemia blast cell
populations from patients with AML. Expression of oncogene protein
products will be determined by immunofluorescence studies using antisera to
the oncogene proteins, and by immunoprecipitation studies of radiolabelled
cell lysates. The aim of these studies is to correlate the expression of
oncogenes with patterns of myeloid cell-surface antigens on normal and
malignant cells to determine whether there is a relationship with
differentiation status. Results will further our understanding of leukemia
cell biology in AML, and are directed towards clarifying the maturational
defect that occurs in myelogenous leukemia. By determining the
maturational capabilities of the L-CFC population, alternative approaches
to therapy, including immunotherapy and maturation-inducing therapy, may
result.
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Monoclonal antibodies to carbohydrate antigens in autologous bone marrow transplantation.
自体骨髓移植中针对碳水化合物抗原的单克隆抗体。
DOI:
10.1002/jcb.240360412
发表时间:
1988
期刊:
Journal of cellular biochemistry
影响因子:
4
作者:
[Ball,ED, Howell,AL]
通讯作者:
Howell,AL
Distribution of the 124-kd antigen defined by monoclonal antibody AML-1-99 on normal and leukemic myeloid cells.
单克隆抗体 AML-1-99 定义的 124-kd 抗原在正常和白血病骨髓细胞上的分布。
DOI:
--
发表时间:
1988
期刊:
Experimental hematology
影响因子:
2.6
作者:
[Howell,AL, Miller,R, Keefe,KA, McIntyre,OR, Stockner,M, Sullivan,R, Hibbert,SR, Noelle,RJ, Ball,ED]
通讯作者:
Ball,ED
Induction of differentiation in blast cells and leukemia colony-forming cells from patients with acute myeloid leukemia.
急性髓性白血病患者的母细胞和白血病集落形成细胞的分化诱导。
DOI:
--
发表时间:
1990
期刊:
Blood
影响因子:
20.3
作者:
[Howell,AL, Stukel,TA, Bloomfield,CD, Davey,FR, Ball,ED]
通讯作者:
Ball,ED
Gamma interferon and 1,25 dihydroxyvitamin D3 cooperate in the induction of monocytoid differentiation but not in the functional activation of the HL-60 promyelocytic leukemia cell line.
γ 干扰素和 1,25 二羟基维生素 D3 共同诱导单核细胞分化,但不参与 HL-60 早幼粒细胞白血病细胞系的功能激活。
DOI:
--
发表时间:
1986
期刊:
Experimental hematology
影响因子:
2.6
作者:
[Ball,ED, Howell,AL, Shen,L]
通讯作者:
Shen,L
Developing novel CRISPR/CasX editors to generate a CCR5/null immune system
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批准号:10553152
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资助金额:$0.0万
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财政年份:2021
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依托单位:
Developing novel CRISPR/CasX editors to generate a CCR5/null immune system
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批准号:10356091
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资助金额:$0.0万
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负责人:ALEXANDRA L HOWELL
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Combination Therapy Using CRISPR/Cas Gene Editing Plus Human Monoclonal Antibodies for a Functional HIV Cure
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批准号:9032718
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项目类别:
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资助金额:$0.0万
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财政年份:2015
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负责人:ALEXANDRA L HOWELL
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依托单位:
Inhibiting Mucosal HIV-1 Transmission by Host Cell RNA Interference
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批准号:7910642
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资助金额:$0.0万
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财政年份:2009
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负责人:ALEXANDRA L HOWELL
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依托单位:
Inhibiting Mucosal HIV-1 Transmission by Host Cell RNA Interference
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批准号:7788891
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项目类别:
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资助金额:$0.0万
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财政年份:2009
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负责人:ALEXANDRA L HOWELL
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依托单位:
Inhibiting Mucosal HIV-1 Transmission by Host Cell RNA Interference
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批准号:8391122
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项目类别:
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资助金额:$0.0万
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财政年份:2009
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负责人:ALEXANDRA L HOWELL
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依托单位:
Inhibiting Mucosal HIV-1 Transmission by Host Cell RNA Interference
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批准号:8195248
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2009
-
负责人:ALEXANDRA L HOWELL
-
依托单位:
CHARACTERIZATION OF ACUTE MYELOGENOUS LEUKEMIA STEM CELL
-
批准号:3446985
-
项目类别:
-
资助金额:$5.63万
-
财政年份:1986
-
负责人:ALEXANDRA L HOWELL
-
依托单位:
CHARACTERIZATION OF ACUTE MYELOGENOUS LEUKEMIA STEM CELL
-
批准号:3446986
-
项目类别:
-
资助金额:$5.34万
-
财政年份:1986
-
负责人:ALEXANDRA L HOWELL
-
依托单位: