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REGULATION OF ANF RELEASE FROM A HEART-LUNG PREPARATION

心肺准备中 ANF 释放的调节

基本信息

批准号：
3449403
负责人：
JOHN R DIETZ
金额：
$ 4.64万
依托单位：
UNIVERSITY OF SOUTH FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
1986
资助国家：
美国
起止时间：
1986-12-01 至 1990-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3449403
关键词：
angiotensins atrial natriuretic peptide blood pressure blood volume dopamine electrolyte balance hormone regulation /control mechanism laboratory rat radioimmunoassay saluresis stretch receptors vasopressins

项目摘要

Recent studies suggest that the atrial natriuretic factor (ANF) is a circulating hormone that may be involved in the regulation blood volume and arterial pressure but little is known concerning the physiological regulation of ANF secretion. One factor which has been shown to release ANF is an increase in atrial stretch or volume. It is important to determine what other factors influence ANF secretion and whether these factors have a direct effect on ANF release or act by modulating the ANF response to atrial distension. For this purpose, a rat heart-lung preparation has been developed in which both venous return and aortic pressure can be regulated and ANF measured directly from the perfusion reservoir by radioimmunoassay. The specific aims of this project are 1) to determine if increases in perfusate sodium concentration have a direct effect on ANF release, 2) to determine if angiotensin II or ADH have a direct negative feedback effect on ANF secretion, 3) to examine the effect of an increase in cardiac afterload on ANF release with venous return held constant, 4) to determine the direct effect of alpha or beta adrenergic receptor stimulation on ANF release under conditions where aortic pressure and venous return are held constant and 5) to test the hypothesis that dopamine plays a role in ANF secretion. It is hypothesized that ANF plays a role in the control of plasma sodium concentration and that angiotensin II and ADH, which are released under conditions of reduced blood volume, will have an inhibitory effect on ANF secretion. Increases in cardiac afterload should increase ANF release by increasing atrial pressure. Although some work suggests that adrenergic stimulation increases ANF release in vivo, it is possible that beta or alpha adrenergic stimulation has a direct inhibitory effect on ANF secretion. Since dopamine antagonists attenuate the natriuresis and hypoaldosteronism produced by volume expansion, it is predicted that dopamine plays a role in ANF release from the heart. These studies will be important in our understanding of the physiological role of ANF in body fluid balance.

最近的研究表明，心房钠尿因子（ANF）是一种可能参与调节血容量的循环激素，动脉压，但很少有人知道有关的生理调节ANF分泌。有一个因素被证明可以释放 ANF是心房牵张或容积的增加。重要的是要确定哪些其他因素影响ANF分泌，以及这些因素是否因子对ANF的释放有直接影响或通过调节ANF起作用对心房扩张的反应。为此，一个大鼠心肺已经开发了一种制剂，其中静脉回流和主动脉回流都是通过静脉回流和主动脉回流来实现的。压力可以调节，ANF可以直接从灌注测量放射免疫分析法检测储库。该项目的具体目标是：（1）确定灌注液钠浓度的增加是否具有直接的对ANF释放的影响，2）确定血管紧张素II或ADH是否具有对ANF分泌的直接负反馈作用，3）检查作用心钠素释放时心脏后负荷增加，静脉回流暂停常数，4）确定α或β肾上腺素能的直接作用在主动脉压升高的条件下，和静脉回流保持不变，5）为了检验假设，多巴胺在ANF分泌中起作用。据推测，胜利阵线发挥作用，在控制血浆钠浓度中的作用， II和ADH，它们在血容量减少的条件下释放，将对ANF分泌具有抑制作用。心血管疾病增加后负荷应通过增加心房压力来增加ANF释放。虽然一些研究表明，肾上腺素能刺激增加ANF 在体内释放，β或α肾上腺素能刺激可能对心钠素分泌有直接抑制作用。自从多巴胺拮抗剂可减弱由以下因素引起的尿钠排泄和醛固酮减少症：体积膨胀，预测多巴胺在ANF释放中起作用发自内心的这些研究对我们理解心钠素在体液平衡中的生理作用。