CARDIAC PEPTIDES IN THE CONTROL OF BLOOD VOLUME

描述：(改编自《摘要》)多年来它 已经很明显，对动脉压的调节密切相关 与体内盐分和水分的平衡有关。最近，有证据表明， 心房含有一种有效的利钠药和降压作用 已被称为心钠素(ANF)的物质。这个 这个项目的长期目标是更好地了解 心钠素等潜在心脏激素在心脏功能调节中的作用 肾功能、血容量和血压。最近的证据表明 除了心钠素，其他由前心钠素分子形成的多肽 在血浆中循环。具体地说，前ANF(31-67)存在于血浆中 比ANF浓度更高，可能有类似的生理效应 致ANF。本项目的目标是：1)建立正常的基线 正常、低盐和高盐摄入大鼠的心钠素原(31-67)值，2) 测定5%、10%或20%血容量扩张对血浆的影响 麻醉大鼠心钠素原(31-67)的变化及其与变化的比较 在ANF中，3)比较相同输注剂量的ANF和 心钠素前体(31-67)对肾功能、血浆肾素活性和血液的影响 压力，4)检测前ANF(31-67)对肾血流、GFR、 肾脏氯化钠排泄、水分排泄和肾素分泌 离体灌流肾，5)比较前-ANF分泌(1-98)， 心钠素原(31-67)和心钠素(99-126)对以下物质的反应： 心房舒展和b)心房起搏的变化以及6)测试a ANF或PRO ANF(31-67)各自的短环负反馈效应 通过测量前-ANF(1-98)的分泌作为 孤立的心房。 尽管许多研究都集中在ANF上，但显然更多的 我们需要的是关于 前ANF分子在控制肾功能和血容量中的作用。近期 有证据表明，这些其他心房肽，特别是心钠素前体 (31-67)，可能具有重要的生理作用。

DESCRIPTION: (Adapted from the Abstract) Over a period of many years it has become apparent that the regulation of arterial pressure is intimately linked to body salt and water balance. Recently, it has been shown that the atria contain a potent natriuretic and blood pressure lowering substance which has been termed the atrial natriuretic factor (ANF). The long-term goals of this project are to obtain a better understanding of the role of ANF and other potential cardiac hormones in the regulation of kidney function, blood volume and blood pressure. Recent evidence suggests that in addition to ANF, other peptides formed from the pro-ANF molecule circulate in plasma. Specifically, pro-ANF (31-67) is present in plasma in higher concentrations than ANF and may have physiological effects similar to ANF. The goals of this project are: 1) to establish normal baseline values for pro-ANF (31-67) in rats on normal, low and high NaCl intakes, 2) to determine the effect of 5, 10 or 20% blood volume expansions on plasma pro-ANF (31-67) in anesthetized rats and compare these changes to changes in ANF, 3) to compare the effects of similar infused doses of ANF and pro-ANF (31-67) on renal function, plasma renin activity and blood pressure, 4) to examine the effect of pro-ANF (31-67) on renal flow, GFR, sodium chloride excretion, water excretion and renin secretion in the isolated perfused kidney, 5) to compare the secretion of pro-ANF (1-98), pro-ANF (31-67) and ANF (99-126) from isolated atria in response to: a) changes in atrial stretch and b) atrial pacing and 6) to test for a short-loop negative feedback effect of ANF or pro-ANF (31-67) on their own production by measuring pro-ANF (1-98) secretion as an end point in the isolated atria. Although numerous studies have focused on ANF, it is apparent that more information is needed on the relative importance the other portions of the pro-ANF molecule in the control of renal function and blood volume. Recent evidence suggests that these other atrial peptides, particularly pro-ANF (31-67), may have an important physiological role.