CARDIAC PEPTIDES IN THE CONTROL OF BLOOD VOLUME

强心肽控制血容量

• 批准号: 3363639
• 负责人: JOHN R DIETZ
• 金额: $ 10.9万
• 依托单位: UNIVERSITY OF SOUTH FLORIDA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-01 至 1994-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3363639
• 关键词: angiotensin /renin /aldosterone hypertension; antihypertensive agents; atrial natriuretic peptide; blood volume; coronary disorder; electrolyte balance; hormone regulation /control mechanism; isolation perfusion; kidney circulation; kidney function; laboratory rat; renin; salt intake; stretch receptors; stroke

DESCRIPTION: (Adapted from the Abstract) Over a period of many years it has become apparent that the regulation of arterial pressure is intimately linked to body salt and water balance. Recently, it has been shown that the atria contain a potent natriuretic and blood pressure lowering substance which has been termed the atrial natriuretic factor (ANF). The long-term goals of this project are to obtain a better understanding of the role of ANF and other potential cardiac hormones in the regulation of kidney function, blood volume and blood pressure. Recent evidence suggests that in addition to ANF, other peptides formed from the pro-ANF molecule circulate in plasma. Specifically, pro-ANF (31-67) is present in plasma in higher concentrations than ANF and may have physiological effects similar to ANF. The goals of this project are: 1) to establish normal baseline values for pro-ANF (31-67) in rats on normal, low and high NaCl intakes, 2) to determine the effect of 5, 10 or 20% blood volume expansions on plasma pro-ANF (31-67) in anesthetized rats and compare these changes to changes in ANF, 3) to compare the effects of similar infused doses of ANF and pro-ANF (31-67) on renal function, plasma renin activity and blood pressure, 4) to examine the effect of pro-ANF (31-67) on renal flow, GFR, sodium chloride excretion, water excretion and renin secretion in the isolated perfused kidney, 5) to compare the secretion of pro-ANF (1-98), pro-ANF (31-67) and ANF (99-126) from isolated atria in response to: a) changes in atrial stretch and b) atrial pacing and 6) to test for a short-loop negative feedback effect of ANF or pro-ANF (31-67) on their own production by measuring pro-ANF (1-98) secretion as an end point in the isolated atria. Although numerous studies have focused on ANF, it is apparent that more information is needed on the relative importance the other portions of the pro-ANF molecule in the control of renal function and blood volume. Recent evidence suggests that these other atrial peptides, particularly pro-ANF (31-67), may have an important physiological role.

摘要：（摘自摘要）多年来， 动脉压的调节与血压的变化密切相关， 与身体盐和水平衡有关。 最近的研究表明， 心房含有有效利尿钠和降低血压 心钠素是一种被称为心房利钠因子（ANF）的物质。 的 该项目的长期目标是更好地了解 心钠素和其他潜在的心脏激素在调节 肾功能血容量和血压 最近的证据表明 除了ANF，由前ANF分子形成的其他肽 在血浆中循环。 具体地，pro-ANF（31-67）存在于受试者的血浆中。 浓度高于ANF，可能具有类似的生理效应 在ANF。 本计画之目标为：1）建立正常基线 大鼠在正常、低和高NaCl摄入量下的pro-ANF（31-67）值，2） 为了确定5、10或20%血容量扩张对血浆的影响， pro-ANF（31-67），并将这些变化与 在ANF中，3）比较相似输注剂量的ANF和 pro-ANF（31-67）对肾功能、血浆肾素活性和血液流变学的影响 压力，4）检查前ANF（31-67）对肾流量，GFR， 氯化钠排泄、水排泄和肾素分泌 5）比较前心钠素（1-98）的分泌， 来自分离的心房的pro-ANF（31-67）和ANF（99-126）响应于： 心房牵张的变化和B）心房起搏和6）测试a ANF或pro-ANF自身的短环负反馈效应（31-67） 通过测量前ANF（1-98）分泌作为终点， 孤立心房 虽然许多研究都集中在ANF上，但很明显， 需要关于其他部分的相对重要性的信息， 前心钠素分子在肾功能和血容量的控制。 最近 有证据表明，这些其他心房肽，特别是前ANF， （31-67），可能具有重要的生理作用。