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CUTANEOUS DETERMINANTS OF RESISTANCE TO LEISHMANIA

抵抗利什曼原虫的皮肤决定因素

基本信息

批准号：
3445466
负责人：
M SUZANNE GIANNINI
金额：
$ 6.18万
依托单位：
COLUMBIA UNIV NEW YORK MORNINGSIDE
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-09-01 至 1986-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3445466
关键词：
Langerhans' cell Leishmania T lymphocyte host organism interaction immunochemistry leishmaniasis leukocyte activation /transformation microorganism immunology protozoal antigen skin ultraviolet radiation

项目摘要

The objective of these studies is to evaluate the role of the cutaneous accessory cells in host resistance to leishmaniasis. These macrophage-like cells form an anastomosing network of antigen-presenting cells in skin, the body's first line of defense against agents of Leishmaniasis. Preliminary studies have shown that antigen-presenting macrophages in epidermal cell suspensions selectively take up Leishmania parasites to which the host can mount an effective immune response. The first phase of the project will examine the ability of cutaneous accessory cells, collected from normal and infected mouse skin, to present parasite antigen to T-cells in vitro. Results will be extended to a murine in vivo model to investigate the role of cutaneous accessory cells in the induction of protective immunity to cutaneous leishmaniasis. In the second phase, accessory cell function will be selectively inactivated and the effect of this treatment on parasite antigen presentation in vitro and on the resolution of cutaneous leishmaniasis in vivo will be evaluated. The third phase will evaluate the ability of cutaneous accessory cells, normally bypassed during infection by intravascular inoculation, to process and present antigen from the agent of visceral leishmaniasis, a parasite to which the host develops little or no immunity when infected. Results from these experiments will be applicable to the understanding of pathogenesis in leishmaniasis, and to the development of effective vaccines. Results may also contribute to our understanding of the role of cutaneous antigen-presenting cells in infectious diseases.

这些研究的目的是评估皮肤的作用，辅助细胞在宿主抗利什曼病中的作用这些巨噬细胞样细胞在皮肤中形成抗原呈递细胞的坏死网络，身体对抗利什曼病的第一道防线初步研究表明，表皮细胞中的抗原呈递巨噬细胞悬浮液选择性地吸收宿主可以吸收的利什曼原虫产生有效的免疫反应该项目的第一阶段将检测皮肤辅助细胞的能力，从正常和感染的小鼠皮肤，在体外将寄生虫抗原呈递给T细胞。结果将扩展到小鼠体内模型，以研究在诱导保护性免疫中的作用皮肤黑热病在第二阶段，辅助细胞功能将选择性灭活，这种治疗对寄生虫的影响体外抗原呈递和皮肤免疫反应的解决将评价体内利什曼病。第三阶段将评估皮肤辅助细胞的能力，通常在感染过程中被旁路，血管内接种，以处理和呈递来自病原体的抗原，内脏利什曼病，一种寄生虫，宿主很少或根本不发育感染时的免疫力。这些实验的结果将适用于对利什曼病发病机制的理解，研制有效的疫苗。结果也可能有助于我们了解皮肤抗原呈递细胞在传染病