KARYOTYPE ANALYSIS OF LEISHMANIA

拟议研究的主要目标是调查 新大陆种巴西利什曼原虫的染色体组构 和L.墨西哥。 这些生物体引起了广泛的人类疾病 并感染各种各样的载体和储存宿主。 的 两个物种各自细分为几个亚种，基于 生物学和流行病学特征，但遗传的程度 每个亚种内的多样性和它们之间的关系是 未知 通过脉冲场凝胶（PFG）电泳获得的初步数据显示， 来自利什曼原虫个别地理分离株的种群 染色体大小的特征分布（PFG核型）。 因此 不同群体的PFG核型大小程度较高 只有当种群属于同一亚种时才能保持一致性。 关于PFG 核型分析，我们将确定是否染色体一致性发生在 同一亚种的种群。 这一分析将扩展到 大湖墨西哥情结。 研究结果将使我们深入了解遗传信息是如何被 组织在利什曼尼亚，从而提供有关的基本信息， 生物体的生物学。 新大陆的遗传多样性 将对利什曼原虫种及其亚种进行评价， 他们之间的关系得到了澄清。 PFG核型分析可应用于 分离株的分类，因此结果与临床相关 诊断和流行病学。

The principal goal of the proposed research is to investigate the chromosomal organization of the New World species Leishmania braziliensis and L. mexicana. These organisms cause a broad spectrum of human diseases and infect an extraordinary variety of vectors and reservoir hosts. The two species are each subdivided into several subspecies, based on biological and epidemiological features, yet the extent of genetic diversity within each subspecies and the relationships among them are unknown. Preliminary data obtained by pulsed field gel (PFG) electrophoresis show that stocks derived from individual geographic isolates of Leishmania have characteristic distributions of chromosome sizes (PFG karyotypes). Thus far, PFG karyotypes of different stocks have a high degree of size concordance only when stocks belong to the same subspecies. From PFG karyotyping we will determine whether chromosomal concordances occur among stocks of the same subspecies. This analysis will then be extended to the larger L. mexiciana complex. Results will give insight into the way in which genetic information is organized in Leishmania, thereby providing basic information about the biology of the organisms. The genetic diversity of the New World leishmanial species and their subspecies will be evaluated and the relationships among them clarified. PFG karyotyping can be applied to classification of isolates, and thus the results are relevant to clinical diagnosis and epidemiology.