THE EVALUATION AND BANKING OF ABORTED FETAL TISSUES

流产胎儿组织的评估和储存

• 批准号: 3450356
• 负责人: DAVID WARE BRANCH
• 金额: $ 26.94万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1993-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3450356
• 关键词: chromosome aberrations; ectopic pregnancy; embryo /fetus tissue /cell culture; epidemiology; gene mutation; hematopoietic stem cells; hematopoietic tissue; human fetus tissue; human tissue; natural gene amplification; pregnancy loss; spontaneous abortion; tissue /cell culture; tissue /organ preservation; tissue resource /registry; urinalysis

We propose to develop a large, comprehensive bank of placental and fetal specimens from spontaneous abortions and ectopic pregnancies, which includes detailed clinical data (and parental specimens whenever possible). Fetal and placental tissues (and RNA or DNA extracted from them) will be essential for our ongoing research into the causes of early pregnancy loss. Ultimately, the material collected may prove to be a valuable source of stem cells for fetal tissue transplantation (especially in Utah where relatively few pregnancies are terminated "electively"). Our multi-disciplinary team will exhaustively characterize specimens in the bank for the prevalence and significance of known and suspected etiologic factors. A drug recall history and a urine toxicology screen will be obtained from every consenting woman. Embryonic and fetal tissues will be analyzed for chromosomal abnormalities will undergo a detailed teratology/dysmorphology examination, and will be cultured for relevant bacteria and viruses. DNA amplification techniques will also be used as a complimentary approach to screen the tissues for microorganisms. Polymorphic DNA markers will be used to compare maternal, paternal, and fetal samples to screen for genetic events which are not normally apparent on routine cytogenetic examination (such as isodisomy, microdeletions, and single gene mutations) Finally, we will test the feasibility of obtaining and culturing fetal hematopoietic progenitors using fetal liver from spontaneous abortions and ectopic pregnancies. The success or failure of these cultures will be compared with our success in culturing neonatal hematopoietic progenitors and will be correlated with the clinical data and the laboratory investigations. To prolong their availability of stem cells for transplantation, we will attempt long-term culture and cryopreservation of the hematopoietic progenitors.

我们建议建立一个大的，全面的胎盘和胎儿库， 自然流产和异位妊娠的标本， 包括详细的临床数据（以及可能的亲本标本）。 胎儿和胎盘组织（以及从中提取的RNA或DNA）将被 这对我们正在进行的关于早孕流产原因的研究至关重要。 最终，收集到的材料可能会成为一个有价值的来源， 用于胎儿组织移植的干细胞（特别是在犹他州， “选择性”终止妊娠的情况相对较少）。我们 多学科团队将详尽描述标本中的 已知和疑似病因的患病率和重要性数据库 因素 药物召回史和尿液毒理学检查将 从每一个自愿的女人那里得到的。 胚胎和胎儿组织将 染色体异常分析将进行详细的 畸形学/畸形学检查，并将进行相关培养 细菌和病毒。 DNA扩增技术也将被用作 一种筛选组织中微生物的补充方法。 多态性DNA标记将用于比较母亲，父亲， 胎儿样本筛查通常不明显的遗传事件 常规细胞遗传学检查（如同二体性、微缺失和 单基因突变）最后，我们将测试获得 以及使用来自人的胎肝培养胎儿造血祖细胞， 自然流产和异位妊娠。 的成败 这些培养物将与我们成功培养新生儿的 造血祖细胞，并将与临床数据和 实验室调查。 为了延长他们获得干细胞的时间 对于移植，我们将尝试长期培养和冷冻保存 造血祖细胞。