FETAL STEM CELLS FOR TRANSPLANTATION

胎儿组织移植一直被认为是一种治疗方法。 各种危及生命的情况。胎儿细胞可能是唯一适合 用于移植，因为：(1)它们的生长和增殖能力， (2)细胞和组织分化的能力(固有的 可塑性)，(3)它们产生生长因子的能力，以及(4)它们 与成体细胞相比，抗原性降低。如果恰当和合乎道德地 获得人工流产似乎是一个巨大的来源 可移植的细胞和组织在这个国家。然而，人工流产 组织，以及愿意捐赠组织的女性，还没有 详尽描述以确定它们是否适合 移植目的。此外，最优的收集方法， 处理、评估、维护和储存胎儿组织和细胞 线路尚未确定。我们的团队主要感兴趣的是 胎儿造血干细胞用于宫内移植 治疗先天性代谢性疾病、免疫缺陷和血液病 精神错乱。我们建议对人工流产组织和 捐献组织的妇女是否适合胎儿组织 移植。我们计划使用以前为 自然流产的研究和目前用于表征的 人工流产标本中细菌和病毒的检测 污染、形态异常和细胞遗传学异常。 我们将测试我们是否使用DNA技术来搜索传染性 基因病因可能更有效，也可能更敏感 而不是传统的微生物学和细胞遗传学技术。我们也会 确定产量、生长潜力、细胞类型和免疫学 培养的造血干细胞的特性。最后，我们还将 确定培养的细胞是否可以超低温保存、运输、 并移植到成熟的宫内移植模型中。

Transplantation of fetal tissues has been advocated as a means of treating various life-threatening conditions. Fetal cells may be uniquely suited for transplantation because of: (1) their ability to grow and proliferate, (2) their ability to undergo cell and tissue differentiation (intrinsic plasticity), (3) their ability to produce growth factors, and (4) their reduced antigenicity compared to adult cells. If properly and ethically obtained, induced abortions would seem a voluminous source of transplantable cells and tissues this country. However, induced abortion tissues, and the women who would donate the tissues, have not been exhaustively characterized to determine if they are suitable for transplantation purposes. In addition, the optimal methods for collecting, processing, evaluating, maintaining, and banking fetal tissues and cell lines has not been determined. Our group is primarily interested in the use of fetal hematopoietic stem cells for in utero transplantation to treat inborn errors of metabolism, immune deficiencies, and hematologic disorders. We propose to characterize induced abortion tissues and the women donating the tissues with regard to suitability for fetal tissue transplantation. We plan to use protocols previously developed for the study of spontaneous abortions and currently in use to characterize induced abortion specimens with respect to bacterial and viral contamination, morphological abnormalities, and cytogenetic abnormalities. We will test whether our use of DNA technology to search for infectious and genetic etiologies may be more efficient and perhaps more sensitive than traditional microbiologic and cytogenetic techniques. We also will determine the yield, growth potential, cell types, and immunologic characteristics of cultured hematopoietic stem cells. Finally we will also determine whether the cultured cells can be cryopreserved, transported, and transplanted in a well-established in utero transplantation model.