FETAL STEM CELLS FOR TRANSPLANTATION

用于移植的胎儿干细胞

• 批准号: 2403426
• 负责人: DAVID WARE BRANCH
• 金额: $ 26.92万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2403426
• 关键词: Chlamydiaceae; Herpesviridae disease; bacterial disease; cell type; chromosome aberrations; cryopreservation; cytokine; hematopoietic stem cells; hematopoietic tissue transplantation; high performance liquid chromatography; human fetus tissue; human tissue; in situ hybridization; induced abortion; information systems; polymerase chain reaction; questionnaires; virus diseases

Transplantation of fetal tissues has been advocated as a means of treating various life-threatening conditions. Fetal cells may be uniquely suited for transplantation because of: (1) their ability to grow and proliferate, (2) their ability to undergo cell and tissue differentiation (intrinsic plasticity), (3) their ability to produce growth factors, and (4) their reduced antigenicity compared to adult cells. If properly and ethically obtained, induced abortions would seem a voluminous source of transplantable cells and tissues this country. However, induced abortion tissues, and the women who would donate the tissues, have not been exhaustively characterized to determine if they are suitable for transplantation purposes. In addition, the optimal methods for collecting, processing, evaluating, maintaining, and banking fetal tissues and cell lines has not been determined. Our group is primarily interested in the use of fetal hematopoietic stem cells for in utero transplantation to treat inborn errors of metabolism, immune deficiencies, and hematologic disorders. We propose to characterize induced abortion tissues and the women donating the tissues with regard to suitability for fetal tissue transplantation. We plan to use protocols previously developed for the study of spontaneous abortions and currently in use to characterize induced abortion specimens with respect to bacterial and viral contamination, morphological abnormalities, and cytogenetic abnormalities. We will test whether our use of DNA technology to search for infectious and genetic etiologies may be more efficient and perhaps more sensitive than traditional microbiologic and cytogenetic techniques. We also will determine the yield, growth potential, cell types, and immunologic characteristics of cultured hematopoietic stem cells. Finally we will also determine whether the cultured cells can be cryopreserved, transported, and transplanted in a well-established in utero transplantation model.

胎儿组织移植已被提倡作为一种治疗手段 各种危及生命的情况。胎儿细胞可能特别适合 用于移植是因为：(1) 它们具有生长和增殖的能力， （2）它们进行细胞和组织分化的能力（内在的 可塑性），（3）它们产生生长因子的能力，以及（4）它们的 与成体细胞相比，抗原性降低。如果正确且合乎道德 获得的，人工流产似乎是一个巨大的来源 这个国家的可移植细胞和组织。然而，人工流产 组织以及愿意捐赠组织的妇女尚未得到 详尽地描述以确定它们是否适合 移植目的。此外，收集的最佳方法， 处理、评估、维护和储存胎儿组织和细胞 线路尚未确定。我们小组主要感兴趣的是 使用胎儿造血干细胞进行宫内移植 治疗先天性代谢缺陷、免疫缺陷和血液学缺陷 失调。我们建议表征人工流产组织和 捐赠组织的妇女考虑胎儿组织的适用性 移植。我们计划使用之前开发的协议 自然流产的研究，目前用于表征 人工流产标本中的细菌和病毒 污染、形态异常和细胞遗传学异常。 我们将测试我们是否可以利用DNA技术来寻找传染性病毒 遗传病因学可能更有效，也可能更敏感 优于传统的微生物学和细胞遗传学技术。我们也会 确定产量、生长潜力、细胞类型和免疫学 培养的造血干细胞的特性。最后我们也会 确定培养的细胞是否可以冷冻保存、运输、 并移植到完善的子宫移植模型中。

项目成果

Transfusion of fetal cord blood cells: an improved method of hematopoietic stem cell transplantation?

胎儿脐带血细胞输注：造血干细胞移植的改进方法？

• DOI: 10.1016/s0165-0378(98)00040-0
• 发表时间: 1999
• 期刊: Journal of reproductive immunology
• 影响因子: 3.4
• 作者: Craven,CM;Ward,K
• 通讯作者: Ward,K

Placental causes of fetal malformation.

胎儿畸形的胎盘原因。

• DOI: 10.1097/00003081-199609000-00008
• 发表时间: 1996
• 期刊: Clinical obstetrics and gynecology
• 影响因子: 1.5
• 作者: Craven,C;Ward,K
• 通讯作者: Ward,K

Angiotensinogen T235 expression is elevated in decidual spiral arteries.

蜕膜螺旋动脉中血管紧张素原 T235 表达升高。

• DOI: 10.1172/jci119661
• 发表时间: 1997
• 期刊: The Journal of clinical investigation
• 作者: Morgan,T;Craven,C;Nelson,L;Lalouel,JM;Ward,K
• 通讯作者: Ward,K

Hypervolemia of pregnancy is not maintained in mice chronically overexpressing angiotensinogen.

长期过度表达血管紧张素原的小鼠不能维持妊娠期高血容量。

• DOI: 10.1016/j.ajog.2006.04.005
• 发表时间: 2006
• 期刊: American journal of obstetrics and gynecology
• 影响因子: 9.8
• 作者: Morgan,TerryK;Rohrwasser,Andreas;Zhao,Ling;Hillas,Elaine;Cheng,Tong;Ward,KennethJ;Lalouel,Jean-Marc
• 通讯作者: Lalouel,Jean-Marc

The factor V Leiden mutation is not a common cause of recurrent miscarriage.

莱顿 V 因子突变并不是反复流产的常见原因。

• DOI: 10.1016/s0165-0378(97)00039-9
• 发表时间: 1997
• 期刊: Journal of reproductive immunology
• 影响因子: 3.4
• 作者: Dizon-Townson,DS;Kinney,S;Branch,DW;Ward,K
• 通讯作者: Ward,K