NERVOUS AND HUMORAL CONTROL OF RENAL RESISTANCE ARTERIES

肾阻力动脉的神经和体液控制

• 批准号: 3449184
• 负责人: MARY P OWEN
• 金额: $ 3.83万
• 依托单位: COLLEGE OF MEDICINE AT ROCKFORD
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3449184
• 关键词: angiotensin II; cardiac output; hormone regulation /control mechanism; kidney circulation; prostaglandins; renal hypertension; vascular resistance; vascular smooth muscle

The aim of this proposal is to study the characteristics of resistance arteries in the kidney and their regulation by certain neural (noradrenergic and possibly dopaminergic and cholinergic) and humoral (angiotensin and prostaglandin) control mechanisms. Relatively little is known from direct in vitro experimentation of this important class of vessels (resistance arteries) and virtually nothing concerning those arteries located in the kidney. Functional features of the innervation, smooth muscle and endothelium of rabbit renal arteries as small as 50 MuI.D. will be documented by the utilization of a resistance vessel myograph and structural and biochemical measurements. These features will be related to vessel diameter and/or location in the kidney. The specific objectives of research include: (1) determination of how nmoradrenergic control varies with artery diameter and/or kidney location, (2) pharmacological classification and functional description of rabbit renal vascular dopamine receptors, (3) determination of the presence of a rabbit renal dopaminergic innervation and investigation of the possibility of a neurogenic vasodilator mechanism involving dopamine, (4) determination of the presence of an endothelial-dependent acetylcholine mediated vasodilation response inversely proportional to vessel diameter and investigation of the possibility of a neurogenic vasodilator mechanism in renal arteries involving acetylcholine and (5) pharmacological and functional description of the direct response to angiotensin in renal arteries. Specific objectives 1 and 5 require some delineation of the modulation of vasoconstrictor influences in the renal arteries by endogeneous prostaglandins and determination of the dependency of that modulation on an intact endothelium. The overall objective is to understand changes that occur in vessel characteristics with change in their diameter and/or location in the kidney. Although resistance arteries in general play a pivotal role in circulatory function and appear to be altered in hypertension, only recently have direct studies of any of these vessels been undertaken in vitro. In spite of the fact that renal blood flow constitute 15-25% of cardiac output and is therefore of general circulatory interest, this represents the first detailed in vitro study to be undertaken in renal resistance arteries.

这项建议的目的是研究耐药性的特点 肾脏中的动脉及其由某些神经系统调节 （去甲肾上腺素能和可能的多巴胺能和胆碱能）和体液 （血管紧张素和前列腺素）控制机制。 之甚少 从这类重要的化合物的直接体外实验已知， 血管（阻力动脉）和几乎没有关于这些 位于肾脏的动脉。 神经支配的功能特征， 小至50 多D将通过使用阻力容器记录 肌电图和结构及生化测量。 这些功能将 与肾脏中的血管直径和/或位置相关。 具体 研究的目的包括：（1）确定nmoradrenergic 控制随动脉直径和/或肾脏位置而变化，（2） 兔肾的药理分类和功能描述 血管多巴胺受体，（3）确定是否存在兔 肾多巴胺能神经支配和研究的可能性， 神经源性血管扩张机制涉及多巴胺，（4）确定 内皮依赖性乙酰胆碱介导的存在 血管舒张反应与血管直径成反比， 神经源性血管扩张机制的可能性的研究 涉及乙酰胆碱的肾动脉和（5）药理学和 肾血管紧张素直接反应的功能描述 动脉 具体目标1和5需要对 调节肾动脉中的血管收缩剂影响， 内源性甘草素及其依赖性的测定 对完整内皮的调节。 总体目标是 了解血管特征随 它们的直径和/或在肾脏中的位置。 虽然阻力动脉 通常在循环功能中起关键作用， 在高血压中的改变，直到最近才有直接的研究， 血管在体外进行。 尽管肾血 血流量占心输出量的15-25%，因此一般 这是第一个详细的体外研究， 在肾阻力动脉中进行。