EFFECT OF ZINC AND COPPER IMBALANCE ON SERUM LIPIDS

锌和铜不平衡对血清脂质的影响

• 批准号: 3448945
• 负责人: DENIS M MEDEIROS
• 金额: $ 4.88万
• 依托单位: UNIVERSITY OF WYOMING
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-01-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3448945
• 关键词: adolescence (12-20); blood lipid; blood lipoprotein; caloric dietary content; cardiovascular disorder epidemiology; cholesterol; clinical chemistry; copper; developmental nutrition; dietary supplements; dietary trace element; hypocupremia; male; metal metabolism disorder; nutrition related tag; serum; triglycerides; urine; young adult human (21-34); zinc

The primary objective of the proposed research is to study the effect of oral zinc supplementation upon serum triglycerides, total cholesterol, and lipoprotein-cholesterol fractions, and blood pressure in young white males. A secondary objective is to study the effect of oral zinc supplementation upon serum, hair, and urine zinc and copper concentration. Three groups of white males (12-15 in each group), ages 18-29 years, will receive either 50 mg zinc as zinc sulfate, 75 mg zinc as zinc sulfate, or gelatin capsules, for twelve weeks. Subjects will be unaware of the treatment they are receiving. Three day diet records will be completed by subjects biweekly after supplementation begins. Food samples will also be collected at the same time periods. Diet records and food samples will be analyzed for daily intakes of calories, protein, carbohydrate, fat, polyunsaturated:saturated fat ratio, as well as copper and zinc intake. Hair, a 24 hr urine sample, and overnight fasting serum sample will be taken from each subject before supplementation and at various time intervals there-after. Hair, urine, and serum will be analyzed for copper and zinc concentrations. Hair and serum data will be expressed in parts per million, whereas urine data will be expressed as ng Cu or Zn per gm creatinine. Food samples will also be analyzed for copper, zinc, and calcium concentrations. Water samples will also be analyzed for the same elements. Throughout the study, subjects will be maintained on a diet low in copper, zinc, phytate, and fiber to avoid interferences in zinc absorption. Diet histories of all subjects will be collected at the beginning of the study. A repeated measures design (split plot through time) will be used to analyze the effect of zinc supplementation upon dependent variables. Covariant adjustment for age, weight/height, and calorie intake will be made. Data on the intake of other dietary components other than zinc supplementation are affecting results.

拟议研究的主要目标是研究 口服补锌对血清甘油三酯、总胆固醇和 年轻白人的脂蛋白-胆固醇组分和血压 男性。第二个目标是研究口服锌的效果。 补充血清、头发和尿锌和铜的浓度。 三组白人男性(每组12-15人)，年龄18-29岁，将 接受50毫克锌作为硫酸锌，75毫克锌作为硫酸锌，或 明胶胶囊，疗程12周。受试者将不会意识到 他们正在接受治疗。三天的饮食记录将在 受试者在补充开始后每两周服用一次。食物样本也将被 在同一时间段收集的。饮食记录和食物样本将 分析每日卡路里、蛋白质、碳水化合物、脂肪的摄入量 多不饱和：饱和脂肪比例，以及铜和锌的摄入量。 头发、24小时尿样和隔夜空腹血清样本 在补充前和不同时间取自每个受试者 在那之后的间歇期。头发、尿液和血清中的铜将被分析 和锌浓度。头发和血清数据将分部分表示 每百万人，而尿液数据将以每克铜或锌为单位 肌酸酐。食品样本也将被分析铜、锌和 钙浓度。水样也将进行同样的分析 元素。在整个研究过程中，受试者将保持低饮食 在铜、锌、植酸盐和纤维中，避免锌的干扰 吸收。所有受试者的饮食历史将在 研究的开始。重复测量设计(拆分地块 时间)将用于分析补锌对健康的影响 因变量。对年龄、体重/身高和 卡路里的摄入量将会增加。关于其他饮食摄入量的数据 锌补充剂以外的其他成分正在影响结果。