CELL BIOLOGY IMMUNOLOGY ON ANIMAL MODELS FOR VITILIGO

这里提出的研究的长期目标是 对血管内皮细胞瘤发生的病理过程的高级知识 白癜风是一种皮肤和眼睛的色素细胞 被毁了。这些实验的目的是为了分析 两种白癜风动物模型的广泛色素脱失 研究了Smyth鸡(以前称为大坝 鸡)和一种新的哺乳动物，小鼠模型(Vit/Vit)。两个人 应采取的方法涉及基本黑素细胞缺陷和 随后的自身免疫反应。无论是色素还是 免疫系统将不得不被协调一致地操纵在 从以下结果可以看出的任何治疗方案 建议的研究。 这些实验包括对黑化的深入研究 Smyth系，在脉络膜黑素细胞和神经管中均有 衍生的黑素细胞在培养过程中出现故障 与在体内观察到的黑素细胞相似。此外, 试图纠正Smyth的异常行为 提出了体外和体内的黑素细胞。休闲装 异常黑化与选择性黑化的关系 黑素小体的自噬细胞反应也将被研究。 Smyth鸡在不同时期获得的血清 将对白癜风的发育阶段进行筛查 黑素细胞特异性抗体。这样的抗体将被用来 定位黑素细胞特异性抗原 电子显微镜下的免疫细胞化学。寻找…… 皮肤与脉络膜黑素细胞特异性表面抗原的比较 将通过建立各自的纯文化来启动 黑素细胞类型。此外，在形态和组织化学方面 VIT/VIT小鼠毛球和眼部色素脱失分析 将启动以及抗黑素细胞因子的筛选 在这个过早变灰的突变株系的血清中 黑素细胞培养的发展。

The long-term objective of the research proposed here is to advance knowledge of the pathologic processes occurring in vitiligo, a disease in which pigment cells of the skin and eyes are destroyed. The experiments are aimed at analyzing the basis for depigmentation in two animal models for vitiligo: the extensively studied Smyth chicken (previously referred to as the DAM chicken) and a new mammalian, murine model (vit/vit). The two approaches to be taken concern the basic melanocytic defect and the subsequent autoimmune response. Both the pigmentary and the immune systems will have to be manipulated in concert during any therapeutic regimen that might be indicated by the results of the proposed research. The experiments include in-depth studies of melanization in the Smyth line, both within chororidal melanocytes and in neural-tube derived melanocytes that malfunction in culture in a manner similar to that observed in melanocytes in vivo. In addition, attempts to rectify the aberrant behavior of Smyth melanocytes, both in vitro and in vivo, are proposed. The casual relationship between aberrant melanization and the selective authophasgocytosis of melanosomes will also be investigated. Serum from Smyth chickens obtained during various developmental stages of the vitiligo will b screened for melanocyte-specific antibodies. Such antibodies will then be used to localize the melanocyte-specific antigens immunocytochemically by electron microscopy. A search for cutaneous versus choroidal melanocyte-specific surface antigens will be initiated by establishing pure cultures of the respective melanocyte types. In addition, morphologic and histochemical analysis of hair bulb and eye depigmentation in the vit/vit mouse will be initiated as well as screening for anti-melanocyte factors in the serum of this prematurely graying mutant line and development of melanocyte cultures.