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PHENOLS/CATECHOLS IN OCCUPATIONAL/CONTACT VITILIGO SKIN

职业/接触性白癜风皮肤中的酚类/儿茶酚

基本信息

批准号：
6532985
负责人：
RAYMOND E BOISSY
金额：
$ 25.57万
依托单位：
UNIVERSITY OF CINCINNATI
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-08-01 至 2004-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6532985
关键词：
antioxidants apoptosis catechols cytotoxicity environmental toxicology gene expression guinea pigs hazardous substances human tissue melanocyte occupational hazard pathologic process phenols tissue /cell culture vitiligo work site

项目摘要

DESCRIPTION: Complexion coloration is essential to ones health, self-image, and thus overall wellness, both physically and psychologically. Cutaneous pigmentation protects a person from various environmental assaults, like ultraviolet light, as well as potential cellular injury, that can cause cancer and aging of the skin. Loss of skin pigmentation can result in cancer, aging, and compromised immunity of the skin as well as psychological and social problems of self- esteem and personal interactions. Occupational/contact vitiligo is a disease that results in the loss of cutaneous pigmentation. This form of vitiligo occurs relatively frequently in individuals exposed to phenolic/catecholic derivatives primarily in the work place. This disease can be financially and socially devastating for the individual, in addition to it compromising the productivity in the workplace. The general goal of this proposal is to assess the pathological effects of phenolic and catecholic derivatives on melanocytes. These prevalent chemical toxins are responsible for the development of occupational/contact vitiligo in the skin of some individuals exposed to these environmental agents in the workplace. Specifically, we propose to assess the relative cytotoxicity, the interaction with tyrosinase, and the generation of toxic free radical products within melanocytes exposed to various phenolic and catecholic derivatives. In this assessment, we will determine whether melanocytes from all patients with vitiligo, patients with the occupational/contact form of vitiligo, family members of patients with vitiligo, and/or a subset of the population in general, are more sensitive to cytotoxicity by these phenolic/catecholic derivatives. In addition, we will assess [1] genes that are differentially expressed and [2] regulators of apoptosis in cells treated with phenolic/catecholic derivatives. In addition, we will determine whether the response of these molecules is altered in vitiligo melanocytes demonstrated to be more susceptible to cytotoxicity. Finally, we will assess the effectiveness of various antioxidants, especially catalase, in dampening the cytotoxic effect of phenolic/catecholic derivatives using cultured melanocytes, organotypic cultures of human skin, and a guinea pig model.

产品说明：补色对一个人的健康、自我形象，身体和心理上的全面健康。皮肤色素沉着保护人们免受各种环境的侵害，比如紫外线，以及潜在的细胞损伤，这可能会导致癌症和衰老的皮肤皮肤色素沉着的丧失会导致癌症、衰老和受损。皮肤的免疫力以及自我的心理和社会问题，尊重和个人互动。白癜风是什么病？导致皮肤色素的丧失。白癜风的症状有哪些在接触酚类/儿茶酚类物质的个体中相对频繁发生衍生品主要在工作场所。这种疾病可以在经济上和社会破坏性的个人，除了它损害了工作场所的生产力。本提案的总体目标是评估酚类和儿茶酚类衍生物对黑素细胞的作用。这些常见的化学物质毒素是负责职业性/接触性白癜风的发展，一些人的皮肤暴露于这些环境因素中，职场具体而言，我们建议评估相对细胞毒性，与酪氨酸酶相互作用，并产生有毒的自由基产物在暴露于各种酚和儿茶酚衍生物的黑素细胞中。在在这项评估中，我们将确定所有患有黑色素瘤的患者的黑色素细胞是否白癜风，职业性/接触性白癜风患者，家庭白癜风患者的成员，和/或一般来说，对这些酚类/儿茶酚类化合物的细胞毒性更敏感，衍生物. 此外，我们还将评估[1]差异表达的基因，表达和[2]调节细胞凋亡的细胞处理，酚/儿茶酚衍生物。此外，我们将确定这些分子的反应在白癜风黑素细胞中改变，对细胞毒性更敏感。最后，我们将评估各种抗氧化剂，特别是过氧化氢酶，在抑制酚类/儿茶酚衍生物的细胞毒性作用黑素细胞、人类皮肤的器官型培养物和豚鼠模型。