CELL BIOLOGY/IMMUNOLOGY MODELS FOR VITILIGO

在这里提出的研究的长期目标是推进 对白癜风发生的病理过程的认识 皮肤和眼睛的哪些色素细胞被破坏。这些实验 旨在分析两种动物模型中色素脱失的基础 白癜风：经过广泛研究的Smyth鸡(以前曾提到 作为母鸡)和一种新的哺乳动物，小鼠模型(vi/vi)。两个人 应采取的方法涉及基本的黑素细胞缺陷和 随后是自身免疫反应。无论是色素还是免疫性 在任何治疗过程中，系统都必须被协调操作 拟议的研究结果可能表明了这一养生法。 这些实验包括对Smyth系列黑化的深入研究， 在脉络膜黑素细胞和神经管来源的黑素细胞中 在培养中出现故障的方式与观察到的 体内的黑素细胞。异常黑化之间的因果关系 黑素小体的选择性自噬作用也将是 调查过了。Smyth鸡在不同时期获得的血清 白癜风的发育阶段，将进行筛查 黑素细胞特异性抗体。这样的抗体将被用来 黑素细胞特异性抗原的免疫细胞化学定位 显微镜。皮肤与脉络膜黑素细胞特异性对比研究 表面抗原将通过建立细菌的纯培养来启动 不同的黑素细胞类型。此外，毛发的形态分析 将启动vi/vi小鼠的球状和眼睛脱色以及 早产儿血清中抗黑素细胞因子的筛选 变灰的突变系。

The long-term objective of the research proposed here is to advance knowledge of the pathologic processes occurring in vitiligo, a disease in which pigment cells of the skin and eyes are destroyed. The experiments are aimed at analyzing the basis for depigmentation in two animal models for vitiligo: the extensively studied Smyth chicken (previously referred to as the DAM chicken) and a new mammalian, murine model (vi/vi). The two approaches to be taken concern the basic, melanocytic defect and the subsequent, autoimmune response. Both the pigmentary and the immune systems will have to be manipulated in concert during any therapeutic regimen that might be indicated by the results of the proposed research. The experiments include in-depth studies of melanization in the Smyth line, both within choroidal melanocytes and in neural-tube derived melanocytes that malfunction in culture in a manner similar to that observed in melanocytes in vivo. The causal relationship between aberrant melanization and the selective autophagocytosis of melanosomes will also be investigated. Serum from Smyth chickens obtained during various developmental stages of the vitiligo, will be screened for melanocyte-specific antibodies. Such antibodies will then be used to localize the melanocyte-specific antigens immunocytochemically by electron microscopy. A search for cutaneous versus choroidal melanocyte-specific surface antigens will be initiated by establishing pure cultures of the respective melanocyte types. In addition, morphologic analysis of hair bulb and eye depigmentation in the vi/vi mouse will be initiated as well as screening for anti-melanocyte factors in the serum of this prematurely graying mutant line.