CHONDROGENIC REPAIR IN CARTILAGE DEFECTS

(改编自调查人员摘要)关节软骨长期以来 在遭受创伤性侮辱后恢复能力有限或 退行性关节疾病。用来修复软骨损伤的概念 同种异体移植材料可以追溯到一个多世纪以前。使用可行的 软骨细胞作为一种移植材料，其结果好坏参半 文学。该小组的初步研究得出了一个模型 用于修复兔的软骨损伤。这项技术利用 已在体外培养的软骨细胞在随后的 自体移植。这是一个内在软骨愈合的模型。 因为造成的缺陷不会侵犯软骨下板。 拟议的几组实验将利用此模型来增加 愈合的数量和质量，并提高手术疗效和 程序的重复性。巩固和避免分散 移植的细胞，软骨细胞将在胶原海绵或 凝胶。这些复合移植物将被自动移植到 新西兰白兔的膝盖骨。移植物中的软骨细胞将 被打上无线电标签。动物将在很短的时间间隔内被宰杀 8周来确定修复机制并注意细胞的变化 扩散。在这项实验的同时，针对 将制备I型和II型胶原并用于免疫定位 胶原蛋白基质。 第三组实验将调查一种更受控制和 可重复使用的材料用于原位固定移植物。这个 前面描述的模型使用骨膜来实现这一目的。一系列 不同的合成和自然产生的材料将被测试 他们有能力在受到强烈压力的情况下保持贪污 联合环境。第四个实验将评估长期的命运 这些自体移植的移植物。动物将被成群结队地献祭 至术后一年。在牺牲的时候，这些移植物将 通过定量组织学，广泛的生物力学测试进行评估， 并对T细胞活化进行染色。移植物将在以下时间之前进行放射性标记 以监测这些移植细胞的长期命运。 这项工作的临床应用将保证使用同种异体移植物 而不是自动移植的材料。因此，一组类似的实验 对上一次的自体移植进行检查，以确定是否 同种异体复合移植物将产生可接受的结果。相同 将利用技术，但重点将放在监测上 是一种免疫反应。

(Adapted from investigator's abstract) Articular cartilage has long been shown to have a limited capacity for healing following traumatic insult or degenerative joint disease. The concept of healing cartilage lesions with allograft materials goes back over a century. The use of viable chondrocytes as a graft material has had mixed results as reported in the literature. Preliminary studies by this group have resulted in a model for healing cartilage lesions in the rabbit. This technique utilizes chondrocytes which have been culture in vitro prior to subsequent autotransplantation. This is a model for intrinsic cartilage healing because the defect which is made does not violate the subchondral plate. The proposed sets of experiments will utilize this model to increase the amount and quality of healing and to increase the surgical efficacy and reproducibility of the procedure. To consolidate and avoid dispersion of grafted cells, chondrocytes will be grown in either collagen sponges or gels. These composite grafts will be autotransplanted into defects in the patellae of New Zealand White rabbits. The chondrocytes in the graft will be radiolabeled. Animals will be sacrificed in short time intervals up to eight weeks to ascertain the mechanism of repair and note changes in cell proliferation. Concurrent to this experiment, monoclonal antibodies to type I and II collagen will be prepared and used to immunolocalize collagen matrix. A third set of experiments will investigate a more controlled and reproducible material to use for fixation of the graft in situ. The previously described model uses periosteum for this purpose. A series of different synthetic and naturally-occurring materials will be tested for their ability to hold a graft in place within the vigorously stressed joint environment. A fourth experiment will evaluate the long-term fate of these autotransplanted grafts. Animals will be sacrificed in groups up to one year postoperatively. At the time of sacrifice, these grafts will be evaluated by quantitative histology, extensive biomechanical testing, and stained for T-cell activation. Grafts will be radiolabeled prior to transplantation to monitor the long-term fate of these graft cells. Clinical application of this work would warrant the use of allografted instead of autografted materials. Therefore, a set of experiments similar to the preceding one for autografts will be conducted to ascertain whether allografted composite grafts will give an acceptable result. The same techniques will be utilized but emphasis will be placed on the monitoring of an immune response.