MOLECULAR ANALYSIS OF AN INTERNAL IMAGE AUTOANTIBODY

使用从内部图像导出的主序列信息 自身抗体使呼肠孤病毒3型细胞得以识别 附件站点。这个系统提供了一个学习的机会 一种抗菌素的分子特征和功能活性 淋巴细胞自身抗体，类似于某些自身免疫中的抗体 疾病。 合成肽将被用来探索特定氨基酸的作用 与受体接触所涉及的酸残基。站点定向 呼肠孤病毒3型血凝素(HA3)基因的突变将是 在该地区开展了一项活动，以确认和推广这些成果。装订 与呼肠孤病毒3型受体(Reo3R)以及中和 结合细胞的抗呼肠病毒3型单抗(9BG5) 病毒的附着部位，将进行研究。这些研究将允许 病毒细胞自身抗体仿制的分子特征 附件站点。 结合呼肠孤病毒的中和性单抗9BG5 类型3和自身抗体87.92.6也将被研究。 9BG5株核苷酸及推导的氨基酸序列测定 将允许开发与其结合的合成肽类似物 地点。这些多肽可用于一系列研究 研究呼肠孤病毒3型、独特型之间的各种相互作用 (9BG5)、抗独特型自身抗体和Reo3R。这将允许 分子表征的内在相互作用 抗独特型自身抗体的发展。

Use of primary sequence information derived from an internal image autoantibody has allowed the identification of the reovirus type 3 cell attachment site. This system provides an opportunity to study the molecular characteristics and functional activities of an anti- lymphocyte autoantibody, similar to those implicated in some autoimmune diseases. Synthetic peptides will be utilized to probe the role of specific amino acid residues involved in contacting the receptor. Site directed mutagenesis of the reovirus type 3 hemagglutinin (HA3) cDNA will be carried out in this region to confirm and extend these results. Binding to the reovirus type 3 receptor (Reo3R), as well as to a neutralizing anti-reovirus type 3 monoclonal antibody (9BG5) which binds the cell attachment site of the virus, will be studied. These studies will allow molecular characterization of autoantibody mimicry of a viral cell attachment site. The neutralizing monoclonal antibody 9BG5, which binds both reovirus type 3 and the autoantibody 87.92.6, will also be studied. Determination of the nucleotide and deduced amino acid sequences of 9BG5 will allow the development of synthetic peptide analogs of its binding site. These peptides can be utilized in a series of studies to investigate the various interactions between reovirus type 3, idiotype (9BG5), anti-idiotypic autoantibody, and the Reo3R. This will allow the molecular characterization of the interactions inherent in the development of anti-idiotypic autoantibodies.