MOLECULAR ANALYSIS OF AN INTERNAL IMAGE AUTOANTIBODY

内部图像自身抗体的分子分析

• 批准号: 3455258
• 负责人: WILLIAM V WILLIAMS
• 金额: $ 11.55万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3455258
• 关键词: antibody formation; antiidiotype antibody; autoantibody; blocking antibody; immunoglobulin structure; laboratory mouse; lymphocyte; microorganism hemagglutinin; monoclonal antibody; nucleic acid probes; protein sequence; protein structure function; receptor binding; site directed mutagenesis; synthetic peptide; virus receptors

Use of primary sequence information derived from an internal image autoantibody has allowed the identification of the reovirus type 3 cell attachment site. This system provides an opportunity to study the molecular characteristics and functional activities of an anti- lymphocyte autoantibody, similar to those implicated in some autoimmune diseases. Synthetic peptides will be utilized to probe the role of specific amino acid residues involved in contacting the receptor. Site directed mutagenesis of the reovirus type 3 hemagglutinin (HA3) cDNA will be carried out in this region to confirm and extend these results. Binding to the reovirus type 3 receptor (Reo3R), as well as to a neutralizing anti-reovirus type 3 monoclonal antibody (9BG5) which binds the cell attachment site of the virus, will be studied. These studies will allow molecular characterization of autoantibody mimicry of a viral cell attachment site. The neutralizing monoclonal antibody 9BG5, which binds both reovirus type 3 and the autoantibody 87.92.6, will also be studied. Determination of the nucleotide and deduced amino acid sequences of 9BG5 will allow the development of synthetic peptide analogs of its binding site. These peptides can be utilized in a series of studies to investigate the various interactions between reovirus type 3, idiotype (9BG5), anti-idiotypic autoantibody, and the Reo3R. This will allow the molecular characterization of the interactions inherent in the development of anti-idiotypic autoantibodies.

使用从内部图像导出的主要序列信息 自身抗体可以鉴定呼肠孤病毒3型细胞 附着位点 该系统提供了一个研究 的分子特征和功能活性， 淋巴细胞自身抗体，类似于那些涉及一些自身免疫性 疾病 合成肽将被用来探测特定氨基酸的作用， 与受体接触的酸性残基。 定点 呼肠孤病毒3型血凝素（HA3）cDNA的诱变将是 在该地区进行，以确认和扩展这些结果。 结合 呼肠孤病毒3型受体（Reo3R），以及中和 结合细胞的抗呼肠孤病毒3型单克隆抗体（9BG 5） 将研究病毒的附着位点。 这些研究将使 病毒细胞自身抗体模拟的分子特征 附着位点 中和性单克隆抗体9BG 5，其结合呼肠孤病毒 3型和自身抗体87.92.6也将被研究。 9BG 5基因核苷酸序列和推导的氨基酸序列的测定 将允许开发其结合的合成肽类似物， 绝佳的价钱 这些肽可用于一系列研究， 研究呼肠孤病毒3型、独特型 （9 BG 5）、抗独特型自身抗体和Reo 3R。 这将允许 分子表征的相互作用固有的 抗独特型自身抗体的发展。