MOLECULAR ANALYSIS OF AN INTERNAL IMAGE AUTOANTIBODY

内部图像自身抗体的分子分析

• 批准号: 3455260
• 负责人: WILLIAM V WILLIAMS
• 金额: $ 11.38万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3455260
• 关键词: antibody formation; antiidiotype antibody; autoantibody; blocking antibody; immunoglobulin structure; laboratory mouse; lymphocyte; microorganism hemagglutinin; monoclonal antibody; nucleic acid probes; protein sequence; protein structure function; receptor binding; site directed mutagenesis; synthetic peptide; virus receptors

Use of primary sequence information derived from an internal image autoantibody has allowed the identification of the reovirus type 3 cell attachment site. This system provides an opportunity to study the molecular characteristics and functional activities of an anti- lymphocyte autoantibody, similar to those implicated in some autoimmune diseases. Synthetic peptides will be utilized to probe the role of specific amino acid residues involved in contacting the receptor. Site directed mutagenesis of the reovirus type 3 hemagglutinin (HA3) cDNA will be carried out in this region to confirm and extend these results. Binding to the reovirus type 3 receptor (Reo3R), as well as to a neutralizing anti-reovirus type 3 monoclonal antibody (9BG5) which binds the cell attachment site of the virus, will be studied. These studies will allow molecular characterization of autoantibody mimicry of a viral cell attachment site. The neutralizing monoclonal antibody 9BG5, which binds both reovirus type 3 and the autoantibody 87.92.6, will also be studied. Determination of the nucleotide and deduced amino acid sequences of 9BG5 will allow the development of synthetic peptide analogs of its binding site. These peptides can be utilized in a series of studies to investigate the various interactions between reovirus type 3, idiotype (9BG5), anti-idiotypic autoantibody, and the Reo3R. This will allow the molecular characterization of the interactions inherent in the development of anti-idiotypic autoantibodies.

使用源自内部图像的一级序列信息 自身抗体已允许鉴定 3 型呼肠孤病毒细胞 附件站点。 该系统提供了一个学习的机会 抗病毒药物的分子特征和功能活性 淋巴细胞自身抗体，类似于某些自身免疫性疾病中涉及的抗体 疾病。 合成肽将用于探测特定氨基的作用 参与接触受体的酸残基。 现场定向 呼肠孤病毒 3 型血凝素 (HA3) cDNA 的诱变将 在该地区进行的研究是为了确认和扩展这些结果。 装订 呼肠孤病毒 3 型受体 (Reo3R) 以及中和剂 结合细胞的抗呼肠孤病毒 3 型单克隆抗体 (9BG5) 将研究病毒的附着位点。 这些研究将允许 病毒细胞自身抗体模拟的分子特征 附件站点。 中和单克隆抗体 9BG5，可结合两种呼肠孤病毒 3型和自身抗体87.92.6也将被研究。 9BG5的核苷酸和推导的氨基酸序列的测定 将允许开发其结合的合成肽类似物 地点。 这些肽可用于一系列研究 研究 3 型呼肠孤病毒、独特型之间的各种相互作用 (9BG5)、抗独特型自身抗体和 Reo3R。 这将允许 固有相互作用的分子表征 抗独特型自身抗体的开发。