BIOCHEMICAL STUDIES OF ROD OUTER SEGMENT PLASMA MEMBRANE

拟议工作的目的是通过以下两个方面来描述两者 生化和EM免疫细胞化学处理蛋白质 脊椎动物杆状外段血浆的组成 薄膜。这项工作的长期目标是确定 视盘过程中是否发生外段蛋白分选 形态发生，从而产生质膜，其 在成分上与光盘不同。基因产品的分类， 作为电池结构和长期维护的一般特征， 可能与缓慢发病的视网膜退行性变有关 与人类的情况非常相似，即视网膜色素变性。这个 提案还涉及到对血浆的识别 参与光敏反应的膜成分 脊椎动物的通透性机制和更新过程 以及它们可能与疾病有关的任何关系 在视盘脱落和吞噬过程中。 识别外段血浆的三种基本策略 提出了膜组件的概念。第一个涉及探测 完整杆状外节段的胞外表面 抗体和凝集素已经可用。第二部分包括 浓缩质膜组分的制备 凝胶电泳法和后续抗体鉴定 制作。第三种策略考察了两种策略之间的相似性 光感受器和其他细胞的胞外表面。全 三种策略都依赖于生化和 免疫细胞化学方法用于确定白血病患者的身份 质膜成分。

It is the intention of the proposed work to characterize both by biochemical and EM immunocytochemical procedures the protein composition of the vertebrate rod outer segment plasma membrane. The long-term objective of the work is to determine whether sorting of outer segment proteins occurs during disc morphogenesis, thereby generating a plasma membrane which differs compositionally from the discs. Sorting of gene products, as a general feature of cell structure and long-term maintenance, may be relevant to slow-onset retinal degenerations which more closely resemble the human condition, retinitis pigmentosa. The proposal also is concerned with the identification of plasma membrane constituents which participate in the light-sensitive permeability mechanism and renewal processes of the vertebrate rod outer segment, and any relation they may have to maladies during disc shedding and phagocytosis. Three basic strategies for identifying outer segment plasma membrane components are proposed. The first involves probing the extracellular surface of intact rod outer segments with antibodies and lectins already available. The second consists of preparing enriched plasma membrane fractions for characterization by gel electrophoresis and subsequent antibody production. The third strategy examines similarities between the extracellular surfaces of photoreceptors and other cells. All three strategies depend upon both biochemical and immunocytochemical evaluations for establishing the identity of plasma membrane constituents.