BIOCHEMICAL STUDIES OF ROD OUTER SEGMENT PLASMA MEMBRANE

拟议工作的意图是， 生化和EM免疫细胞化学方法 脊椎动物视杆细胞外节血浆的组成 膜的 这项工作的长期目标是确定 外节蛋白的分选是否发生在盘 形态发生，从而产生质膜， 与光盘的成分不同。 基因产物的分类， 作为细胞结构和长期维持的一般特征， 可能与慢性视网膜变性有关， 与人类的视网膜色素变性非常相似 的 该提案还涉及血浆的鉴定 膜成分参与了光敏感 脊椎动物的渗透机制和更新过程 杆外节，以及任何关系，他们可能有疾病 在椎间盘脱落和吞噬过程中。 识别外节等离子体的三种基本策略 提出了膜组件。 第一个是探测 完整杆外节的细胞外表面， 抗体和凝集素已经可用。 第二个包括 制备富集的质膜部分， 通过凝胶电泳和随后的抗体表征 生产 第三个策略研究了 光感受器和其他细胞的细胞外表面。 所有 三种策略依赖于生物化学和 免疫细胞化学评价，用于确定 质膜成分。

It is the intention of the proposed work to characterize both by biochemical and EM immunocytochemical procedures the protein composition of the vertebrate rod outer segment plasma membrane. The long-term objective of the work is to determine whether sorting of outer segment proteins occurs during disc morphogenesis, thereby generating a plasma membrane which differs compositionally from the discs. Sorting of gene products, as a general feature of cell structure and long-term maintenance, may be relevant to slow-onset retinal degenerations which more closely resemble the human condition, retinitis pigmentosa. The proposal also is concerned with the identification of plasma membrane constituents which participate in the light-sensitive permeability mechanism and renewal processes of the vertebrate rod outer segment, and any relation they may have to maladies during disc shedding and phagocytosis. Three basic strategies for identifying outer segment plasma membrane components are proposed. The first involves probing the extracellular surface of intact rod outer segments with antibodies and lectins already available. The second consists of preparing enriched plasma membrane fractions for characterization by gel electrophoresis and subsequent antibody production. The third strategy examines similarities between the extracellular surfaces of photoreceptors and other cells. All three strategies depend upon both biochemical and immunocytochemical evaluations for establishing the identity of plasma membrane constituents.