IGA FC RECEPTORS ON MYCOPLASMAS & IMPACT ON DISEASE

支原体上的 IGA FC 受体

• 批准号: 3455447
• 负责人: Mary B Brown
• 金额: $ 10.57万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-02-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3455447
• 关键词: Mycoplasma; antibody receptor; antireceptor antibody; chickens; disease /disorder model; immunoglobulin A; laboratory mouse; model design /development; mycoplasmal pneumonia; protein purification; protein structure function; virulence; western blottings

The long term goal of the proposed research is to determine the role, if any, that IgA Fc receptors on the surface of bacteria play in the pathogenesis of respiratory disease. IgG Fc receptors, most notably Protein A, have been characterized and studied extensively. IgA Fc receptors are less well characterized, and to date have been described in Group A and B streptococci. We have identified several mycoplasmal species which bind human IGA Fc fragments. All of the mycoplasmal species which bound IgA Fc fragments were associated with respiratory disease in their natural host. A major deterrent to determination of the significance of IgA Fc receptors in disease has been the lack of a model system to adequately evaluate the impact of the Fc receptors on microbial pathogenicity. One of the IgA-binding mycoplasma species, Mycoplasma pulmonis, is the etiologic agent of murine respiratory mycoplasmosis (MRM), and is an excellent experimental model for infectious respiratory disease. We will characterize the IgA Fc receptors(s) on M. pulmonis with respect to specificity for immunoglobulin classes and subclasses from different host species. We will determine if mycoplasmas of human origin also express IgA Fc receptors. We will isolate and purify the IgA Fc receptors(s) on M. pulmonis, and determine the biochemical and immunochemical properties of the purified Fc receptor(s). The significance of IgA Fc receptor(s) in respiratory disease will be determined based on expression by strains on M. pulmonis which differ in virulence, expression of Fc receptor after passage in the natural host, and effects on pulmonary clearance after aerosol exposure to IgA-coated M. pulmonis. The character of potential IgAl and IgA2 Fc receptors could represent a potentially useful tool in elucidation of IgA Fc mediated effector functions. Finally, the presence of IgA Fc receptors on the surface of M. pulmonis coupled with the defined parameters for production and quantitation of respiratory disease in the mouse system make this a particularly attractive model to determine the impact of bacterial IgA Fc receptors on respiratory disease.

拟议研究的长期目标是确定作用，如果 任何，伊加Fc受体在细菌表面发挥作用， 呼吸道疾病的发病机制。 IgG Fc受体，最值得注意的是 蛋白A已经被广泛地表征和研究。 伊加FC 受体的特征不太清楚，迄今为止， A群和B群链球菌。 我们已经鉴定了几种支原体 其结合人伊加Fc片段。 所有的支原体种类， 结合的伊加Fc片段与呼吸系统疾病相关， 自然宿主 一个主要的威慑力，以确定的意义， 伊加Fc受体在疾病中一直缺乏一个模型系统， 充分评价Fc受体对微生物 致病性 支原体是IgA结合支原体种属之一， 肺支原体，是小鼠呼吸道支原体病（MRM）的病原体， 是传染性呼吸道疾病的优良实验模型。 我们将表征M.肺结核， 对来自不同宿主的免疫球蛋白类别和亚类的特异性 物种 我们将确定是否人源性支原体也表达伊加 Fc受体。 我们将分离和纯化M上的伊加Fc受体。 肺，并确定生化和免疫化学特性， 纯化的Fc受体。 伊加Fc受体（s）的意义 呼吸道疾病将基于菌株在M上的表达来确定。 不同毒力、传代后Fc受体表达 在自然宿主中的作用，以及气雾剂给药后对肺清除率的影响 暴露于IgA包被的M.肺动脉 潜在IgAl的性质和 IgA2 Fc受体可能是一个潜在的有用的工具，在阐明 伊加Fc介导的效应子功能。 最后，伊加Fc的存在 M.与定义的参数耦合的肺 用于在小鼠系统中产生和定量呼吸系统疾病 使其成为一个特别有吸引力的模型，以确定 细菌伊加Fc受体对呼吸道疾病的影响。