ROLE OF A DNA-PROTEIN COMPLEX IN CLASS II MHC GENE EXPRE

DNA-蛋白质复合物在 CLASS II MHC GENE EXPRE 中的作用

• 批准号: 3455784
• 负责人: Patricia W Finn
• 金额: $ 10.65万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1997-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3455784
• 关键词: DNA binding protein; DNA footprinting; MHC class II antigen; complementary DNA; gel mobility shift assay; gene complementation; gene expression; genetic transcription; human genetic material tag; laboratory rabbit; molecular cloning; protein structure function; tissue /cell culture; transfection

The goal of these studies is to elucidate the mechanisms that regulate major histocompatibility complex (MHC) class II (Ia) gene expression. Class II gene expression is regulated in terms of tissue specificity, stage of development and in response to external stimuli. It has been determined that the regulation of MHC class II gene expression occurs at the level of transcription. We have recently defined several regulatory elements and DNA binding factors for class n genes. We have focused on a novel nuclear binding protein, Complex A, whose presence correlates with class II MHC expression. Our data support the hypothesis that the presence of Complex A plays a critical role in class II gene transcription. To determine the role of Complex A in class II gene expression, we will first determine the structure and function of Complex A in human class II gene expression. Second, to further analyze Complex A, we will exploit the correlation between Complex A and a family of yeast transcription factors. Finally, in order to characterize proteins which regulate class II expression, we will use a functional complementation strategy to isolate cDNA clones which restore MHC class II expression to class II deficient cells.

这些研究的目标是阐明调控机制 主要组织相容性复合体(MHC)II类(Ia)基因表达。 II类基因的表达受组织特异性、分期的调控 对发展和对外部刺激的反应。已经确定了 MHC-II类基因表达的调节发生在 抄写。我们最近定义了几个监管要素，并 N类基因的DNA结合因子。我们关注的是一种新的核能 结合蛋白，复合体A，其存在与第二类MHC相关 表情。我们的数据支持这样的假设，即复合体A的存在 在第二类基因转录中起关键作用。要确定 复合体A在II类基因表达中的作用，我们将首先确定 复合体A在人类II类基因表达中的结构与功能 其次，为了进一步分析复数A，我们将利用相关性 在复合体A和酵母转录因子家族之间。最后，在 为了表征调节第二类基因表达的蛋白质，我们将 使用功能互补策略分离出 将MHC II类表达恢复到II类缺陷细胞。