ETIOLOGY OF LESIONS OF THE ORAL MUCOSA

口腔粘膜病变的病因学

• 批准号: 3462064
• 负责人: KAREN ADLER STORTHZ
• 金额: $ 8.33万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-05-01 至 1992-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3462064
• 关键词: aphthous stomatitis; human papillomavirus; human tissue; laboratory mouse; molecular cloning; neoplasm /cancer; oncogenic virus; oral mucosa; oral pharyngeal neoplasm; viral carcinogenesis

There are many different lesions of the oral mucosa including malignancies of various types that can jeopardize the health of the patient. It is estimated that there will be 30,000 new cases of oral cancer in the United States in 1986. Efforts aimed at identification of possible etiologic agents are clearly warranted. Papillomaviruses are one of the many kinds of viruses found in the mouth. Because of their tropism for epithelium, they are excellent candidates as etiologic agents of benign and malignant lesions of the oral mucosa. This study will investigate the role of human papillomavirus in lesions of the oral mucosa. Tissues from patients with various oral lesions will be analyzed prospectively and retrospectively for evidence of papillomavirus infection. New types of papillomavirus will be cloned where indicated. In vitro studies of cellular transformation will examine the oncogenic potential of the human papillomaviruses found in the mouth. The approaches to these problems will be through direct studies of human tissues by staining with antibodies to papillomavirus antigens and by hybridization to DNA of known types of papillomavirus. To investigate the oncogenicity of human papillomavirus, cultured cells will be transfected with human papillomavirus DNA alone or in combination with other agents such as herpes simplex virus and various chemical carcinogens. Cell lines established from oral cancers will be examined for the presence of human papillomavirus nucleic acid sequences to provide further evidence for the oncogenic potential of these viruses. It is hoped that this study will increase the understanding of the etiology of several types of lesions of the oral mucosa, especially oral malignancies. This research is compatible with research objective FO6 of the National Institute of Dental Research.

口腔粘膜有许多不同的病变，包括恶性肿瘤 可能危害患者健康的各种类型。 是 据估计，美国将有30，000例新的口腔癌病例， 1986年的国家。 旨在确定可能病因的努力 代理人显然是有保证的。 乳头瘤病毒是口腔中发现的多种病毒之一。 由于它们对上皮细胞的向性，它们是作为 口腔粘膜良性和恶性病变的病原体。 这 研究将探讨人乳头瘤病毒在乳腺癌病变中的作用。 口腔粘膜 将从患有各种口腔病变的患者身上采集组织， 前瞻性和回顾性分析乳头瘤病毒的证据， 感染 新类型的乳头状瘤病毒将在指定的地方克隆。 在 细胞转化的体外研究将检查致癌的 口腔中发现的人乳头瘤病毒的潜力。 解决这些问题的方法将是通过直接研究人类 组织用乳头瘤病毒抗原的抗体染色， 与已知类型的乳头瘤病毒的DNA杂交。 探讨 人乳头瘤病毒的致癌性，将培养的细胞转染 单独使用人乳头瘤病毒DNA或与其他药物联合使用 例如单纯疱疹病毒和各种化学致癌物。 细胞系 将检查从口腔癌中建立的人 乳头状瘤病毒核酸序列，以提供进一步的证据， 这些病毒的致癌潜力。 希望本研究能增加对病因的了解 口腔粘膜的几种类型的病变，尤其是口腔 恶性肿瘤。 本研究符合研究目标FO6， 国家牙科研究所