Novel Point-of-Care Diagnostic Techniques for Dementia

这项提议的目的是创造一种新的方法，使用基于石墨烯的复合生物传感器来检测在痴呆症(重点是阿尔茨海默病)的早期阶段生物体液(如血液、脑脊液、尿液和唾液)中发现的生物标记物，以及随着疾病进展水平的变化。一个项目合作伙伴已经开发了一种基于石墨烯传感器的通用护理点(POC)生物传感器技术，并展示了对几种疾病生物标志物的检测。与现有的分析方法不同，该传感器是一个快速诊断平台，在采集样本后几分钟内就能产生实时数据和决定性的测试结果。该项目的一个目标是展示一种基于复合石墨烯生物传感器的新型、低成本、可靠的POC诊断平台，用于早期检测痴呆症并跟踪其在血液等生物液中的进展。开发一种低成本的医疗保健点生物传感技术，使之能够在早期阶段客观地发现痴呆症，并从简单的血液测试中快速监测疾病进展，将彻底改变痴呆症的诊断，并对选择新药临床试验的受试者和监测治疗反应产生巨大的积极影响。

The aim of this proposal is to create a new way, using multiplex graphene-based biosensors, for the detection of biomarkers found in biological fluids (e.g. blood, CSF, urine and saliva) in the early stages of dementia (with emphasis on Alzheimer's disease) and changes in levels with disease progression. A generic, Point-of-Care (POC) biosensor technology, based on graphene transducers, has already been developed by one of the project partners and demonstrated for the detection of several disease biomarkers. Unlike existing analytical approaches, the sensor is a rapid diagnostic platform, yielding real-time data and a conclusive test result within minutes of the sample being taken. The idea is to combine this with novel electronic multiplex techniques and large area graphene to create a multiplexed biosensor array technology to enable the detection of multiple biomarkers of dementia simultaneously with the potential to significantly increase throughput.An objective of the project is to demonstrate a novel, low-cost, reliable, POC diagnostic platform, based on multiplex graphene biosensors, for early detection of dementia and following its progression from biological fluid such as blood. The development of a low cost, point-of-care biosensing technology that makes it possible to detect dementia, objectively, in the early stages and to monitor disease progression, rapidly, from a simple blood test would revolutionise diagnosis of dementia and have a huge positive impact on the selection of subjects for clinical trials for new drugs and for monitoring response to treatment.