EFFECT OF IL-6 AND IL-1 ON HEMOPOIETIC STEM CELLS

IL-6和IL-1对造血干细胞的影响

• 批准号: 3463773
• 负责人: PETER M WONG
• 金额: $ 10.4万
• 依托单位: SUNY DOWNSTATE MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-15 至 1994-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3463773
• 关键词: cell differentiation; cell growth regulation; colony stimulating factor; complementary DNA; gene expression; genetic transcription; gerbil /jird; hematopoietic stem cells; immune response genes; immunogenetics; immunohematology; interleukin 1; interleukin 6; molecular cloning; myeloid stem cell; nucleic acid probes; tissue /cell culture; transfection; transposon /insertion element

Hemopoiesis is regulated by a large family of hemopoietic growth factors or interleukins (IL). Recent accumulating evidence suggests that molecules such as IL-6 and IL-lo, previously thought to act only on more differentiated cells, can also exert an influence on primitive hemopoietic progenitor cells. Whether these molecules have any effect on lymphoid-myeloid stem cells is not known. By developing strategies in transferring and expressing genes coding for these molecules in lymphoid-myeloid stem cells we attempt to study the extent to which they effect growth of these cells. We will also examine whether evolution of a specific lineage of cells from the lymphoid-myeloid stem cells can be determined by 1) tissue specific transcriptional signals placed in front of IL-6 cDNA, and 2) expression of genes coding for lineage- specific hemopoietic growth factors in addition to the inserted IL- 6 gene. These studies will provide important information as to how hemopoietic stem are being regulated by growth factors, singly or in concert with one another.

造血是由一大家族的造血生长调控的