EFFECT OF IL-6 AND IL-1 ON HEMOPOIETIC STEM CELLS

造血是由一个造血生长大家族调节的 因子或白介素类(IL)。最近不断积累的证据 表明像IL-6和IL-10这样的分子，之前认为 只作用于更多分化的细胞，也可以施加 对原始造血祖细胞的影响。不管是这些 分子对淋巴-髓系干细胞没有任何影响 为人所知。 通过制定转移和表达基因的策略 我们在淋巴髓系干细胞中编码这些分子 试图研究它们在多大程度上影响这些 细胞。我们还将研究特定的 淋巴-髓系干细胞的细胞谱系可以是 由1)组织特异性转录信号决定 IL-6基因的表达，以及2)谱系编码基因的表达- 除了插入的IL-1外，还有特定的造血生长因子 6基因。这些研究将提供关于如何 造血干细胞受到生长因子的调节，无论是单一的还是 彼此协调一致。

Hemopoiesis is regulated by a large family of hemopoietic growth factors or interleukins (IL). Recent accumulating evidence suggests that molecules such as IL-6 and IL-lo, previously thought to act only on more differentiated cells, can also exert an influence on primitive hemopoietic progenitor cells. Whether these molecules have any effect on lymphoid-myeloid stem cells is not known. By developing strategies in transferring and expressing genes coding for these molecules in lymphoid-myeloid stem cells we attempt to study the extent to which they effect growth of these cells. We will also examine whether evolution of a specific lineage of cells from the lymphoid-myeloid stem cells can be determined by 1) tissue specific transcriptional signals placed in front of IL-6 cDNA, and 2) expression of genes coding for lineage- specific hemopoietic growth factors in addition to the inserted IL- 6 gene. These studies will provide important information as to how hemopoietic stem are being regulated by growth factors, singly or in concert with one another.