EFFECT OF IL-6 AND IL-1 ON HEMOPOIETIC STEM CELLS

IL-6和IL-1对造血干细胞的影响

• 批准号: 3463774
• 负责人: PETER M WONG
• 金额: $ 11.48万
• 依托单位: SUNY DOWNSTATE MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-15 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3463774
• 关键词: cell differentiation; cell growth regulation; colony stimulating factor; complementary DNA; gene expression; genetic transcription; gerbil /jird; hematopoietic stem cells; immune response genes; immunogenetics; immunohematology; interleukin 1; interleukin 6; molecular cloning; myeloid stem cell; nucleic acid probes; nucleic acid sequence; tissue /cell culture; transfection; transposon /insertion element

Hemopoiesis is regulated by a large family of hemopoietic growth factors or interleukins (IL). Recent accumulating evidence suggests that molecules such as IL-6 and IL-lo, previously thought to act only on more differentiated cells, can also exert an influence on primitive hemopoietic progenitor cells. Whether these molecules have any effect on lymphoid-myeloid stem cells is not known. By developing strategies in transferring and expressing genes coding for these molecules in lymphoid-myeloid stem cells we attempt to study the extent to which they effect growth of these cells. We will also examine whether evolution of a specific lineage of cells from the lymphoid-myeloid stem cells can be determined by 1) tissue specific transcriptional signals placed in front of IL-6 cDNA, and 2) expression of genes coding for lineage- specific hemopoietic growth factors in addition to the inserted IL- 6 gene. These studies will provide important information as to how hemopoietic stem are being regulated by growth factors, singly or in concert with one another.

造血受造血生长大家族调控 因子或白细胞介素（IL）。 最近积累的证据 表明以前认为的IL-6和IL-10等分子 只作用于分化程度更高的细胞， 对原始造血祖细胞的影响。 是否这些 分子对淋巴-骨髓干细胞没有任何影响， 知道的 通过发展转移和表达基因的策略 在淋巴-骨髓干细胞中编码这些分子， 试图研究它们在多大程度上影响这些植物的生长， 细胞 我们还将研究一种特定的 来自淋巴-骨髓干细胞的细胞谱系可以是 由1）组织特异性转录信号决定， IL-6 cDNA的前端，和2）编码谱系- 特异性造血生长因子，除了插入的IL- 6基因。 这些研究将提供重要信息， 造血干细胞受到生长因子单独或 相互协调。