TOXIC FACTORS AFFECTING BONE QUALITY DURING GROWTH

生长过程中影响骨质量的有毒因素

• 批准号: 3465289
• 负责人: Douglas Burke Hanson
• 金额: $ 10.52万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1994-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3465289
• 关键词: beta aminopropionitrile; bone density; cadmium; collagen; crosslink; elasticity; environmental contamination; fluorine; histochemistry /cytochemistry; laboratory rabbit; lead poisoning; neutron diffraction; normal ossification; osteoarthritis; osteogenesis; osteoporosis; toxin

The proposed research focuses on the effect environmental toxins on the material properties of bone during growth. Changes in the macromolecular composition and organization of bone tissue due to toxic substances will be related to alteration in gross architecture of bone. The study will contribute to a general theory of bone properties which will aid in the prevention and treatment of bone disease. Bone of sufficient quality is essential to preventing or forestalling the onset and/or reducing the severity of common adult bone diseases like osteoporosis and osteoarthritis. The ubiquity of environmental toxins make it imperative to understand how bone density, stiffness, collagen fibril structure and the spatial arrangement of bone in cross-section can be affected by these substances. Growing bone is particularly sensitive to environmental insults and reduced bone tissue quality early in life may lead to increased risk of bone disease in adults. It is therefore important to understand the mechanism(s) which control mineralization and elastic properties of bone during growth. Elastic properties, dimensional change with water content, composition, density, neutron diffraction, cross-linking density, and cross-sectional geometric properties are measure to characterize normal and pathological bone at different stages of growth. Bone pathologies are induced under well-controlled conditions by dietary means using beta-aminopropionitrile - - a toxic derivative of the sweet pea, cycads -- a tropical food source containing lethal toxins, and fluoride. Data from physical measurements of the tissue will be combined with data from morphometric and histomorphometric analyses for a comprehensive evaluation of bone pathology induced by toxic substances.

拟议的研究重点是环境毒素对 骨在生长过程中的材料特性。 大分子的变化 骨组织的组成和组织由于有毒物质将是 与骨骼结构的改变有关。 这项研究将 有助于骨特性的一般理论，这将有助于 预防和治疗骨病。 足够质量的骨骼对于预防或阻止 发病和/或降低常见成人骨疾病的严重程度， 骨质疏松症和骨关节炎。 无处不在的环境毒素使得 必须了解骨密度、硬度、胶原纤维 骨在横截面中的结构和空间排列可以 受这些物质的影响。 生长中的骨骼对 在生命早期的环境损伤和降低的骨组织质量可能 导致成年人患骨病风险增加。 因此 重要的是要了解控制矿化的机制， 骨骼在生长过程中的弹性。 弹性性能、尺寸随含水量、成分的变化， 密度、中子衍射、交联密度和横截面 几何特性是表征正常和病理的度量 不同生长阶段的骨骼。 骨病理是在 通过饮食方式使用β-氨基丙腈， - 一种有毒的甜豌豆的衍生物，苏铁--一种热带食物来源 含有致命毒素和氟化物 物理测量数据 组织将与来自形态测量的数据相结合， 骨组织形态计量学分析对骨病理学综合评价 由有毒物质引起的。