SKELETAL MUSCLE ENERGY METABOLISM IN SURGICAL PATIENTS

骨骼肌是身体蛋白质的主要储存库。 在 除了它的功能作用，骨骼肌提供氨基酸， 在压力和营养期间内脏器官的处理 剥夺 热量或蛋白质缺乏可能会导致低于最佳 骨骼肌能量代谢，从而影响肌肉功能， 可以检测到结构或组成的变化。 我们假设休息时肌肉高能量代谢的变化， 在轻度至中度运动期间和运动恢复期间， 用于表征各种营养和代谢状态 与营养消耗和损伤有关。 这些变化可能是 用于了解外科患者营养支持的效果 并确定骨骼肌的最佳营养需求。 将使用磷核磁共振光谱（31 P NMR） 研究营养不良对大鼠高能磷代谢的影响， 骨骼肌。 ATP、磷酸肌酸和 将测量无机磷酸盐，并将无机磷酸盐的复溶速率 在标准工作之前、期间和之后，将对高能池进行监测 载荷 核磁共振技术也将用于测量肌酸激酶 体内活性、pH变化、游离ADP浓度以及 磷酸化潜力。 热量蛋白质缺乏的影响， 再喂养，手术压力和合成代谢激素将被研究。

Skeletal muscle represents the major repository of body protein. In addition to its functional role, skeletal muscle provides amino acids for processing by visceral organs during times of stress and nutrient deprivation. Caloric or protein deficiency may result in less than optimal skeletal muscle energy metabolism thereby affecting muscle function before changes in structure or composition can be detected. We hypothesize that variation in muscle high energy metabolism at rest, during mild to moderate exercise and during recovery from exercise may be used to characterize the various nutritional and metabolic states associated with nutritional depletion and injury. These changes may be used to understand the effects of nutritional support in surgical patients and to determine the optimal nutrient requirements for skeletal muscle. Phosphorus nuclear magnetic resonance spectroscopy (31P NMR) will be used to study the effects of malnutrition on high energy phosphate metabolism in skeletal muscle. The relative quantities of ATP, phosphocreatine, and inorganic phosphate will be measured and the rate of reconstitution of the high energy pool will be monitored before, during and after standard work loads. NMR techniques will also be used to measure creatine kinase activity in vivo, pH changes, free ADP concentrations, and the phosphorylation potential. The effects of caloric protein deprivation, refeeding, operative stress and an anabolic hormone will be studied.