SKELETAL MUSCLE ENERGY METABOLISM IN SURGICAL PATIENTS

手术患者的骨骼肌能量代谢

• 批准号: 3467658
• 负责人: Danny O. Jacobs
• 金额: $ 13.25万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3467658
• 关键词: acid base balance; adenosine triphosphate; bioenergetics; creatine kinase; creatine phosphate; human subject; malnutrition; muscle metabolism; nutrition related tag; phosphates; phosphorylation; postoperative state; protein deficiency; somatotropin; striated muscles

Skeletal muscle represents the major repository of body protein. In addition to its functional role, skeletal muscle provides amino acids for processing by visceral organs during times of stress and nutrient deprivation. Caloric or protein deficiency may result in less than optimal skeletal muscle energy metabolism thereby affecting muscle function before changes in structure or composition can be detected. We hypothesize that variation in muscle high energy metabolism at rest, during mild to moderate exercise and during recovery from exercise may be used to characterize the various nutritional and metabolic states associated with nutritional depletion and injury. These changes may be used to understand the effects of nutritional support in surgical patients and to determine the optimal nutrient requirements for skeletal muscle. Phosphorus nuclear magnetic resonance spectroscopy (31P NMR) will be used to study the effects of malnutrition on high energy phosphate metabolism in skeletal muscle. The relative quantities of ATP, phosphocreatine, and inorganic phosphate will be measured and the rate of reconstitution of the high energy pool will be monitored before, during and after standard work loads. NMR techniques will also be used to measure creatine kinase activity in vivo, pH changes, free ADP concentrations, and the phosphorylation potential. The effects of caloric protein deprivation, refeeding, operative stress and an anabolic hormone will be studied.

骨骼肌是人体蛋白质的主要储存库。在……里面 除了它的功能作用外，骨骼肌还为 内脏在应激和营养状态下的处理 剥夺。卡路里或蛋白质缺乏可能会导致不理想的结果 骨骼肌能量代谢从而影响以前的肌肉功能 可以检测到结构或组成的变化。 我们假设静息状态下肌肉高能量代谢的变化， 在轻度到中度运动期间和在运动后恢复期间， 用来描述各种营养和代谢状态 与营养耗竭和损伤有关。这些变化可能是 用于了解营养支持对外科患者的影响 并确定骨骼肌的最佳营养需求。 将使用磷核磁共振波谱(31P核磁共振) 目的探讨营养不良对高能磷酸盐代谢的影响。 骨骼肌。ATP、磷酸肌酸和肌酸的相对量 无机磷酸盐将被测量和重建的速度 将在标准工作之前、期间和之后对高能池进行监控 大把的。核磁共振技术也将被用于测量肌酸激酶 体内活性、pH变化、游离ADP浓度和 磷酸化潜力。卡路里蛋白质缺乏的影响， 将对再喂养、手术应激和合成激素进行研究。