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METALLOBIOCHEMISTRY OF VANADIUM(V)

钒(V)的金属生物化学

基本信息

批准号：
2180398
负责人：
DEBBIE Catharina CRANS
金额：
$ 9.99万
依托单位：
COLORADO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-04-01 至 1995-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2180398
关键词：
aldehyde lyase biochemistry disease /disorder model electron spin resonance spectroscopy glucose 6 phosphate dehydrogenase glucose metabolism glycolysis glycosylation hexoses insulin sensitivity /resistance isomerase laboratory rat nuclear magnetic resonance spectroscopy organometallic compounds oxidoreductase phosphoglycerate mutase radiotracer vanadium

项目摘要

The chemical and biochemical function of the trace element vanadium is not very well understood. Biological processes such as cardiovascular function, insulin action, the metal ion transport and enzyme inhibition are all affected by vanadium both in vitro and in vivo. This proposal describes studies that will probe the metallobiochemistry of vanadium (V) and the results will be interpreted in terms of a new general mechanism by which vanadium can act. Preliminary experiments carried out in our laboratory suggest that organic vanadate derivatives are accepted as substrates in vitro. The first step in this general mechanism involves spontaneous formation of organic vanadate derivatives. The second step involves enzymes accepting and converting organic vanadate derivatives. The enzyme groups showing these properties include dehydrogenases, isomerases, aldolases, and glycosylases and experiments with individual enzymes in each of these groups will be carried out. The proposed approach involves chemical studies of spontaneous reactions of vanadate with carbohydrates using 51V NMR and CD spectroscopy. Enzyme kinetic methods will be used to determine how effective these vanadate derivatives are as substrates based on Km and Vmax and to probe whether the enzyme source will show any species specific variation that might be related to the reported species tolerances of vanadium. Increased understanding of the available modes of action for vanadium should help to probe its physiological effects such as preventing the cardiovascular decline in diabetic rats. Increased understanding of how vanadium acts may eventually result in vanadate derivatives that could be used biomedically.

微量元素钒的化学和生化功能不是很好理解。心血管等生物过程功能、胰岛素作用、金属离子转运和酶抑制在体外和体内都受到钒的影响。这项建议描述了探索钒(V)的矿物生物化学的研究结果将被解释为一种新的通用机制，其中钒可以起作用。在我们的实验室进行的初步实验实验室建议有机钒酸盐衍生物被接受为体外培养的底物。这一一般机制的第一步包括有机钒酸盐衍生物的自发形成。第二步涉及酶接受和转化有机钒酸盐衍生物。表现出这些特性的酶群包括脱氢酶，异构酶、醛缩酶和糖基酶及个体实验这些组中每一组的酶都将被进行。建议的方法涉及钒与钒的自发反应的化学研究碳水化合物的51V核磁共振和圆二色谱。酶动力学方法将被用来确定这些钒酸盐衍生物作为基于Km和Vmax的底物，并探测酶来源是否会显示任何可能与报告的物种相关的物种特定变异钒的种类容差。更多地了解可用的钒的作用方式有助于探讨其生理效应如预防糖尿病大鼠心血管功能下降等。增加了了解钒的行为可能最终导致钒酸盐的形成。可以用于生物医学的衍生品。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Is vanadate reduced by thiols under biological conditions? Changing the redox potential of V(V)/V(IV) by complexation in aqueous solution.

DOI：
10.1021/ic100080k
发表时间：
2010-05-03
期刊：
INORGANIC CHEMISTRY
影响因子：
4.6
作者：
Crans, Debbie C.;Zhang, Boyan;Gaidamauskas, Ernestas;Keramidas, Anastasios D.;Willsky, Gail R.;Roberts, Chris R.
通讯作者：
Roberts, Chris R.

Effects of metal compounds with distinct physicochemical properties on iron homeostasis and antibacterial activity in the lungs: chromium and vanadium.

DOI：
10.3109/08958370903161232
发表时间：
2010-02
期刊：
Inhalation toxicology
影响因子：
2.1
作者：
Cohen MD;Sisco M;Prophete C;Yoshida K;Chen LC;Zelikoff JT;Smee J;Holder AA;Stonehuerner J;Crans DC;Ghio AJ
通讯作者：
Ghio AJ

(51)V solid-state NMR and density functional theory studies of eight-coordinate non-oxo vanadium complexes: oxidized amavadin.

(51)八配位非氧钒配合物：氧化amavadin的V固态NMR和密度泛函理论研究。

DOI：
10.1039/b820383k
发表时间：
2009
期刊：
Dalton transactions (Cambridge, England : 2003)
影响因子：
0
作者：
Ooms,KristopherJ;Bolte,StephanieE;Baruah,Bharat;Choudhary,MuhammadAziz;Crans,DebbieC;Polenova,Tatyana
通讯作者：
Polenova,Tatyana

Diabetes-altered gene expression in rat skeletal muscle corrected by oral administration of vanadyl sulfate.