ROLE OF CATECHOLAMINES IN THE INITIATION OF LABOR

儿茶酚胺在分娩过程中的作用

• 批准号: 3469456
• 负责人: Charles A Ducsay
• 金额: $ 11.49万
• 依托单位: LOMA LINDA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1991-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3469456
• 关键词: amnion; amniotic fluid; antiadrenergic agents; birth; blood chemistry; catecholamines; catheterization; chorioallantoic membrane; circadian rhythms; dopamine; epinephrine; fatty acid biosynthesis; gestational age; high performance liquid chromatography; hormone regulation /control mechanism; labor complications; muscle contraction; myometrium; norepinephrine; premature infant human; prostaglandin F; radioimmunoassay; statistics /biometry

Premature birth is one of the principal causes of perinatal morbidity and mortality. In order of to aid in the understanding and prevention of this problem, it is imperative to elucidate the factors involved in the regulation of myometrial contractility and the initiation of parturition. Due to ethical considerations, data obtained from human studies are incomplete. This proposal will examine the role of CATS in the initiation of labor by studying the qualitative and quantitative changes in CAT concentrations in the chronically catheterized rhesus monkey. Studies will include measurements of epinephrine, and dopamine in maternal and fetal plasma and amniotic fluid. In addition, PG concentrations (PGEM-II, PGFM, and 6-keto-PGF1alpha) will be quantitated. How these changes are related to alterations in uterine activity and the initiation of labor will also be assessed. In vitro superfusion studies will examine the interaction between CATS and PGs in reproductive tissues collected from pregnant rhesus monkeys. Specifically, what are the stimulatory effects of CATS on PG biosynthesis by decidua, myometrium, amnion, and chorion? PG (PGF2, PGE2 and 6-keto-PGF1alpha) production will be quantitated before and after exposure of the tissue to CATs. These studies will be directed to answer the following questions: 1) Is there a causal relationship between changes in CATS and uterine activity rhythms? 2) Do CATs exert their effects indirectly by first stimulating PG biosynthesis? These studies are in line with the long-term goals of the present research program, namely, to clarify the mechanisms involved in the regulation of uterine contractility and the initiation of labor.

早产是围产期死亡的主要原因之一， 发病率和死亡率。 为了帮助理解 和预防这一问题，必须阐明 参与调节子宫肌层收缩力的因素， 分娩的开始 出于伦理考虑，数据 从人类研究中获得的数据是不完整的。 本提案将审查CATS在启动 通过研究CAT中的质和量的变化来劳动 在长期插管的恒河猴中的浓度。 研究将包括肾上腺素和多巴胺的测量 在母体和胎儿的血浆和羊水中。 此外，PG 浓度（PGEM-II、PGFM和6-keto-PGF 1 α） 定量。 这些变化如何与 还将评估子宫活动和分娩的开始。 体外灌流研究将检查 从妊娠妇女收集的生殖组织中的CATS和PGs 恒河猴 具体来说， CATS对蜕膜、子宫肌层、羊膜和 绒毛膜？ PG（PGF 2、PGE 2和6-keto-PGF 1 α）的产生将 在组织暴露于CAT之前和之后进行定量。 这些研究将回答以下问题： 1)CATS的变化与 子宫活动节律 2)CAT是否发挥了作用 间接地通过首先刺激PG生物合成？ 这些研究 根据本研究计划的长期目标， 也就是说，澄清涉及监管的机制， 子宫收缩力和分娩的开始。