REGULATION OF K+ TRANSPORT IN VASCULAR SMOOTH MUSCLE

这项建议的主题是收缩和收缩之间的关系 血管平滑肌与钾跨膜转运的同步变化 细胞膜。特别是，这次调查的重点是 钾含量增加的特殊离子转运过程 当平滑肌细胞受到刺激时，氯离子会从细胞中转运出来 去收缩。初步数据表明，这种离子的大部分 转运不通过离子通道进行。相反，占主导地位的 钾外流的途径依赖于氯化物和 被环状利尿剂抑制。这两个特征有力地证明了 这条途径代表了某种形式的共运输。研究以确定和 描述了这一运输系统的特点。功能重要性 这个离子转运体的性能将通过评估是否中断 限制了动脉收缩的能力。预赛 研究还表明，细胞内和细胞内的 钙和钾转运过程的激活。因此，决赛 这项研究的阶段将检查细胞内 钙和钾转运过程的激活。因此，决赛 这项研究的阶段将检查细胞内 钙离子浓度和共转运系统的活性。这 目的：评价体外培养的钾离子共转运。 血管平滑肌细胞。这一目标的实现将使 为更详细地研究这种离子运输的规律做准备 系统。这些研究之所以重要和相关，主要有两个原因。 首先，这项研究将推进我们对单价是如何 离子转运系统参与了血管系统的调节。 第二，这些研究直接和直接关系到我们的 对高血压和其他疾病治疗的认识和理论基础 心血管疾病的过程。

The subject of this proposal is the relationship between contraction of vascular smooth muscle and concurrent changes in potassium transport across the cell membrane. In particular, this investigation focuses on the specific ion transport processes responsible for the increase in potassium and chloride transport out of smooth muscle cells when they are stimulated to contract. The preliminary data indicate that the majority of this ion transport does not occur via ion channels. Instead, the predominant pathway for potassium efflux is dependent on the presence of chloride and inhibited by loop diuretics. These two features are strong evidence that this pathway represents some form of cotransport. Studies to identify and characterize this transport system are outlined. The functional importance of this ion transporter will be evaluated by assessing whether interruption of the system limits the ability of arteries to contract. The preliminary studies also demonstrate that there is a tight link between intracellular calcium and activation of the potassium transport process. Thus, the final phase of this study will examine the relationship between intracellular calcium and activation of the potassium transport process. Thus, the final phase of this study will examine the relationship between intracellular calcium concentration and activity of the cotransport system. This objective includes an evaluation of potassium cotransport in cultured vascular smooth muscle cells. Achievement of this objective will set the stage for more detailed studies of the regulation of this ion transport system. The studies are important and relevant for two primary reasons. First, this investigation will advance our understanding of how monovalent ion transport systems participate in regulation of the vascular system. Second, these studies have immediate and direct bearing on our understanding and rationale for treatment of hypertension and other cardiovascular disease processes.