CARDIAC CHLORIDE CURRENT PHARMACOLOGY & PHYSIOLOGY

这项提案中研究的目标是了解更多关于 最近在心肌细胞中发现了氯(Cl)电流。很少 已经知道这种电流，除了它可以在Beta之后被引出- 肾上腺素能刺激，它似乎在 调节动作电位时程。拟议的实验将：(I) 描述这种电流在整个细胞中的生物物理特性 和单通道水平，(Ii)调查通过哪些机制 激动剂和离子调节这一电流的活动，(Iii)确定 已知的阻断氯通道的药物在其他疾病中的作用 以及(Iv)定义氯电流在体内的生理作用 控制心脏的电活动。此信息将 更好地了解心脏的生理调节 功能，并可能为未来的治疗方法提供洞察力 心律不齐。

The goal of the research in this proposal is to learn more about the chloride (Cl) current recently discovered in cardiac myocytes. Very little is known about this current except that it can be elicited following beta- adrenergic stimulation, and it appears to play an important role in regulating action potential duration. The proposed experiments will (i) characterize the biophysical properties of this current at the whole cell and single channel level, (ii) investigate the mechanisms through which agonists and ions regulate the activity of this current, (iii) determine the effect of pharmacologic agents known to block Cl channels in other preparations, and (iv) define the physiological role of the Cl current in controlling electrical activity in the heart. This information will provide a better understanding of the physiological regulation of cardiac function and may provide insight into future approaches to the treatment of arrhythmias.