CARDIAC CHLORIDE CURRENT PHARMACOLOGY AND PHYSIOLOGY

心氯当前药理学和生理学

• 批准号: 3473227
• 负责人: ROBERT D HARVEY
• 金额: $ 6.25万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3473227
• 关键词: G protein; arrhythmia; beta adrenergic receptor; electrical potential; electrophysiology; fluorescence; guinea pigs; heart contraction; heart function; heart pharmacology; laboratory rabbit; phosphorylation

The goal of the research in this proposal is to learn more about the chloride (Cl) current recently discovered in cardiac myocytes. Very little is known about this current except that it can be elicited following beta- adrenergic stimulation, and it appears to play an important role in regulating action potential duration. The proposed experiments will (i) characterize the biophysical properties of this current at the whole cell and single channel level, (ii) investigate the mechanisms through which agonists and ions regulate the activity of this current, (iii) determine the effect of pharmacologic agents known to block Cl channels in other preparations, and (iv) define the physiological role of the Cl current in controlling electrical activity in the heart. This information will provide a better understanding of the physiological regulation of cardiac function and may provide insight into future approaches to the treatment of arrhythmias.

本提案中研究的目标是更多地了解 最近在心肌细胞中发现了氯离子电流。 很少 这是已知的电流，除了它可以引起以下β- 肾上腺素能刺激，它似乎发挥了重要作用， 调节动作电位持续时间。 拟议的实验将（i） 在整个细胞中表征该电流的生物物理特性 和单通道水平，（ii）调查的机制， 激动剂和离子调节该电流的活性，（iii）确定 已知阻断Cl通道的药物在其他细胞中的作用 制剂，和（iv）定义的生理作用的Cl电流， 控制心脏的电活动 这些信息将 更好地了解心脏的生理调节 功能，并可能提供洞察未来的治疗方法， 心律不齐