GROWTH CONE GUIDANCE; INTERACTIONS AND SURFACE MOLECULES

生长锥引导；

• 批准号: 3477152
• 负责人: MICHAEL J BASTIANI
• 金额: $ 8.88万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3477152
• 关键词: Orthoptera; axon; biological models; cell cell interaction; central nervous system; electron microscopy; epithelium; glia; growth cones; laboratory mouse; laboratory rabbit; laboratory rat; monoclonal antibody; neuronal guidance; neurons

The goals of this proposal are to determine (1) what cellular interactions are essential for pioneering the initial scaffold of axon pathways in the embryonic grasshopper central nervous system, (2) how later neurons are guided in their cell specific choices among this scaffold of axon pathways, and (3) what molecules are involved in guiding growth cones in their initial and later pathway choices. The grasshopper embryonic nervous system will be used to answer these questions. This model system is uniquely suited for these experiments because of the in vivo cellular resolution and accessibility it offers. Single identified growth cones in an identified cellular environment can be observed and manipulated in the grasshopper embryo. Specific neuron-epithelial, neuron-glial, and neuron-neuron interactions will be characterized at light and EM level and then experimental manipulations will be performed to determine the role of each interaction in growth cone guidance and pathway choice. Monoclonal antibodies will generated against this same set of epithelial, glial, and neuronal cells to find molecular correlates of the specific interactions required for growth cone guidance.

这项提议的目标是确定(1)什么细胞 互动对于开创 蝗虫胚胎中枢神经的轴突通路 系统，(2)后来的神经元如何在其特定的细胞中被引导 在轴突通路的支架中进行选择，以及(3)什么 分子参与引导生长锥体的初始和 以后的路径选择。蚱蜢的胚胎神经 系统将用来回答这些问题。这个模型系统 特别适合于这些实验，因为在体内 它提供的蜂窝分辨率和可访问性。单一标识 在已识别的蜂窝环境中的生长锥体可以 在蝗虫胚胎中观察和操纵。 特定的神经元上皮细胞、神经元胶质细胞和神经元神经元 相互作用将在光和电磁水平上进行表征，然后 将进行实验操作以确定 每种相互作用在生长锥引导和途径中的作用 选择。将会产生针对该病毒的单抗 一组上皮细胞、神经胶质细胞和神经细胞以寻找分子 生长锥体所需的特定相互作用的相关性 指导。