GROWTH CONE GUIDANCE--INTERACTIONS AND SURFACE MOLECULES

生长锥引导——相互作用和表面分子

• 批准号: 3410682
• 负责人: MICHAEL J BASTIANI
• 金额: $ 25.08万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3410682
• 关键词: Drosophilidae; Orthoptera; cell cell interaction; cell communication molecule; cinemicrography; developmental neurobiology; growth cones; laboratory mouse; laboratory rabbit; monoclonal antibody; nerve /myelin protein; neuronal guidance

Explorers of brain development are still in the age of explosive discovery. New molecules expressed during neurogenesis are being found at an ever increasing rate, and asking how these molecules function together to specify pathways and targets will continue to be our most difficult question. How many different surface molecules contribute to neuronal pathfinding during development and what is the functional contribution that each makes to the dynamic abilities of embryonic neurons? We are beginning to answer these questions by using monoclonal antibodies to isolate new molecules expressed on developing neurons and glia in the grasshopper embryo, and by quantifying the dynamic behaviors and abilities of embryonic neurons using a high resolution time lapse video microscopy system. We are currently characterizing two new molecules, one has been cloned and the other protein has been partially sequenced, and intend to pursue six others with recently isolated monoclonal antibodies. Our goal is to combine these two approaches by developing a novel assay of cellular and molecular function in which we block the expression, or induce ectopic expression, of new molecules and observe differences in the dynamic behaviors of individual neurons in their normal environment.

大脑开发的探索者们还处于爆发时代 的发现 在神经发生过程中表达的新分子正在被 发现的速度越来越快，并询问这些分子如何 将继续共同努力， 是我们最难的问题。 有多少不同的表面 分子在发育过程中有助于神经元寻路 以及每一种对 胚胎神经元的动态能力我们开始回答 通过使用单克隆抗体来分离新的 表达在发育中的神经元和神经胶质细胞上的分子 蝗虫胚胎，并通过量化的动态行为， 胚胎神经元的能力，使用高分辨率的时间推移 视频显微镜系统 我们目前正在描述两个新的 分子，一个已经被克隆，另一个蛋白质已经被克隆。 部分测序，并打算追求其他六个与最近 分离的单克隆抗体 我们的目标是将这两者联合收割机 通过开发一种新的细胞和分子检测方法， 我们阻断表达，或诱导异位表达， 新分子的表达，并观察动态 单个神经元在正常环境中的行为。