MULTIPHOTON IMAGING OF NEMATODE NEURONAL GROWTH CONES

线虫神经元生长锥的多光子成像

• 批准号: 6278475
• 负责人: MICHAEL J BASTIANI
• 金额: $ 2.74万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6278475
• 关键词: Invertebrata; biomedical resource; growth /development; lasers; microscopy; model design /development; nervous system; technology /technique

We want to characterize DD and UD growth cone behavior during embryonic and post embryonic (LI) development of the nematode nervous system. We plan to use multiphoton confocal microscopy and 41) software to observe GFP expressing UD and DD neurons in wild-type and mutant nematodes to better understand the role played by specific molecules in growth cone pathfinding and target recognition. Initial studies will be performed using confocal microscopy. However, it appears likely we will need the multiphoton scope to maintain viability during long term fiequent collection of data sets.

我们想要描述DD和UD生长锥体在 线虫神经的胚胎和胚胎后发育 系统。我们计划使用多光子共聚焦显微镜和41) 用软件观察GFP在野生型和中枢神经元中的表达 更好地了解突变线虫在特定条件下所起的作用 分子在生长锥寻路和目标识别中的作用。首字母 研究将使用共聚焦显微镜进行。然而，它 看来我们将需要多光子望远镜来维持 在长期连续收集数据集期间的可行性。