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PATTERN GENERATION IN SPINAL EXPLANTS

脊柱外植体中的模式生成

基本信息

批准号：
3477101
负责人：
MICHAEL H DROGE
金额：
$ 5.2万
依托单位：
TEXAS WOMAN'S UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1987
资助国家：
美国
起止时间：
1987-09-04 至 1993-08-31
项目状态：
已结题

项目摘要

Due to the complexity of the CNS, the connectivity and communications among individual neurons within pattern generating networks remain undefined. The proposed work will probe the fundamentals of motor rhythms by isolating the smallest possible spinal region still capable of pattern generation. This situation will be approached by explanting a portion of the neonatal mouse spinal cord (with or without the hindlimbs) into an isolated support chamber. Motor activity will be recorded extracellularly from: 1) ventral roots; 2) laminae VII - IX of the spinal cord; and 3) selected muscle nerves. This will include spontaneous activity as well as activity evoked by electrical and pharmacological stimulation. Slice cultures of lumbar spinal segments will also be analyzed for pattern generation. All data will be compared to what is known for intact vertebrates as well as to rhythms that develop in dissociated spinal cell culture. Intracellular recordings will be obtained from neurons identified as either motoneurons or interneurons based on antidromic and dorsal root stimulation tests. Information can then be gathered regarding the synaptic input to identified spinal neurons during rhythmicity. The neurite domains of all intracellularly recorded neurons will be labeled using HRP and mapped in relation to their surrounding circuitry using computerized image processing. This will establish a database of characteristic morphological features that can be correlated with the activity patterns produced under a variety of electrical and pharmacological manipulations. In summary, this proposal is to conduct systematic electrophysiological, pharamacological and morphological analyses of the smallest possible mammalian spinal explants, still capable of generating locomotor-like patterns.

由于CNS的复杂性，模式内单个神经元之间的通信生成网络仍然未定义。拟议的工作将探索运动节律的基本原理，最小可能的脊柱区域仍然能够产生图案。解决这一问题的办法是，新生小鼠脊髓（有或没有后肢）到一个隔离支持室。将记录运动活动细胞外：1）腹根; 2）第VII - IX层的脊髓;和3）选定的肌肉神经。这将包括自发活动以及由电和药理刺激。腰椎组织切片培养还将分析片段以产生图案。所有数据将与已知的完整脊椎动物进行比较在分离的脊髓细胞培养中发展的节奏。将从鉴定的神经元中获得细胞内记录作为运动神经元或中间神经元，背根刺激试验然后可以收集信息关于突触输入到确定的脊髓神经元，节奏性所有细胞内记录的神经突结构域神经元将使用HRP标记，并与它们的周围的电路使用计算机化的图像处理。这将建立一个具有特征的形态特征数据库，这可以与在一个特定环境下产生的活动模式相关联，各种各样的电和药理学操作。在总结一下，这个建议是为了进行系统的电生理学、药理学和形态学对最小可能的哺乳动物脊髓外植体的分析，能够产生类似运动的模式。