PATTERN GENERATION IN SPINAL EXPLANTS

脊柱外植体中的模式生成

• 批准号: 3477098
• 负责人: MICHAEL H DROGE
• 金额: $ 5.22万
• 依托单位: TEXAS WOMAN'S UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-04 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3477098
• 关键词: aminoacid; antidromic impulse; axon; chordate locomotion; electrophysiology; evoked potentials; histochemistry /cytochemistry; histology; image processing; interneurons; laboratory mouse; motor neurons; neuroanatomy; neuropharmacology; neuropil; neurotransmitters; newborn animals; spinal cord; spinal nerves; tissue /cell culture; ventral roots

Due to the complexity of the CNS, the connectivity and communications among individual neurons within pattern generating networks remain undefined. The proposed work will probe the fundamentals of motor rhythms by isolating the smallest possible spinal region still capable of pattern generation. This situation will be approached by explanting a portion of the neonatal mouse spinal cord (with or without the hindlimbs) into an isolated support chamber. Motor activity will be recorded extracellularly from: 1) ventral roots; 2) laminae VII - IX of the spinal cord; and 3) selected muscle nerves. This will include spontaneous activity as well as activity evoked by electrical and pharmacological stimulation. Slice cultures of lumbar spinal segments will also be analyzed for pattern generation. All data will be compared to what is known for intact vertebrates as well as to rhythms that develop in dissociated spinal cell culture. Intracellular recordings will be obtained from neurons identified as either motoneurons or interneurons based on antidromic and dorsal root stimulation tests. Information can then be gathered regarding the synaptic input to identified spinal neurons during rhythmicity. The neurite domains of all intracellularly recorded neurons will be labeled using HRP and mapped in relation to their surrounding circuitry using computerized image processing. This will establish a database of characteristic morphological features that can be correlated with the activity patterns produced under a variety of electrical and pharmacological manipulations. In summary, this proposal is to conduct systematic electrophysiological, pharamacological and morphological analyses of the smallest possible mammalian spinal explants, still capable of generating locomotor-like patterns.

由于CNS的复杂性，连接和 模式内单个神经元之间的通信 发电网络仍未定义。拟议的工作将 通过分离运动节律来探索运动节奏的基本原理 最小的可能的脊椎区域仍然能够产生图案。 解决这种情况的方法是解释 新生小鼠脊髓(带或不带后肢)进入 隔离式支撑室。运动活动将被记录下来 细胞外来源：1)腹根；2)椎板VII-IX 脊髓；3)选定的肌神经。这将包括 自发活动以及由电和电引起的活动 药理刺激。腰椎切片培养 还将分析线束段以生成图案。所有数据 也将与已知的完好的脊椎动物进行比较 关于在分离的脊髓细胞培养中发展的节律。 细胞内的记录将从已识别的神经元中获得 作为运动神经元或中间神经元，基于逆行和 背根刺激试验。然后就可以收集信息了 关于识别的脊髓神经元的突触输入在 节奏性。细胞内记录的All的轴突结构域 神经元将使用HRP进行标记，并根据它们的 使用计算机化图像处理的周围电路。这 将建立一个具有特征的形态特征数据库 它可以与在 各种电学和药理操作。在……里面 综上所述，这项建议是要进行系统的 电生理、药理和形态 对最小的哺乳动物脊椎外植体的分析 能够产生类似运动的图案的。