MOLECULAR ANALYSIS OF RETINAL/PINEAL MELATONIN SYNTHESIS

视网膜/松果体褪黑素合成的分子分析

基本信息

  • 批准号:
    3477955
  • 负责人:
  • 金额:
    $ 7.57万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1990
  • 资助国家:
    美国
  • 起止时间:
    1990-01-01 至 1994-12-31
  • 项目状态:
    已结题

项目摘要

The major goal of this project is to define the structure of the retinal/pineal gene, N-acetyltransferase (NAT) and to examine NAT gene expression in visual and neural transduction. The primary objective of this five year research proposal is to answer the question: Is the retinal/pineal NAT enzyme a key component of a circadian driving oscillator and is the retinal/pineal NAT enzyme controlled by environmental cues at the molecular level? This project will investigate one specific phase of this endogenous rhythm and the entrainment by the light:dark cycle of the rate=limiting enzyme that synthesizes melatonin. The pineal is an excellent system to study all steps in neurochemical transduction, including ligand-receptor interactions, second messenger regulation, and selective regulation of specific genes. Two characteristics for studying molecular mechanisms of pineal neurotransduction are (1) innervation by sympathetic fibers involving alpha- and beta-receptors; and (2) high amplitude circadian rhythms in biochemical activities, including some that are rapidly responsive to known molecular triggers. Although the retina shares similar proteins and biochemical pathways with the pineal, the role of melatonin in the retina is not understood. The experiments will characterize the retinal/pineal tissue-specific genes coding for NAT. Normal tissue-specific expression will be analyzed in retinas and pineals of rats and inbred mice. Primary pineal cell cultures will also be used to study the role adrenergic regulation has in gene expression. The project will have three specific aims: 1. Isolate full-length cDNAs and characterize cDNA probes encoding the retinal/pineal enzyme, NAT, by screening a rat pineal expression library. 2. Generate antisera for immunological probes against recombinant fusion proteins and synthesized peptides identified from the translated cDNAs encoding NAT. 3. Apply these recombinant and immunological probes to localize, characterize and measure changes in circadian regulation and environmental modulation of their mRNA and protein for NAT. The experiments described will employ immunological and recombinant DNA tools to examine the influence of environmental cues on the genes expressed in the pineal and retina. The pineal and retina have been extensively studied by physiological, pharmacological and biochemical approaches. These studies will extend these studies concerning the fundamental molecular nature of circadian rhythms in the nervous system. Biological circadian rhythms are important not only in our daily lives, but in maintaining our health throughout life. Knowledge of the normal structure of genes expressed in the pineal and retina may lead to a better understanding the involvement of the melatonin synthesizing enzyme in visual and neural transduction.
项目的主要目标是定义的结构

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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CHERYL Mae CRAFT其他文献

CHERYL Mae CRAFT的其他文献

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{{ truncateString('CHERYL Mae CRAFT', 18)}}的其他基金

Mechanisms of Cone Pigment Deactivation
锥体颜料失活的机制
  • 批准号:
    7490437
  • 财政年份:
    2005
  • 资助金额:
    $ 7.57万
  • 项目类别:
Mechanisms of Cone Pigment Deactivation
锥体颜料失活的机制
  • 批准号:
    7679415
  • 财政年份:
    2005
  • 资助金额:
    $ 7.57万
  • 项目类别:
Mechanisms of Cone Pigment Deactivation
锥体颜料失活的机制
  • 批准号:
    7279825
  • 财政年份:
    2005
  • 资助金额:
    $ 7.57万
  • 项目类别:
Mechanisms of Cone Pigment Deactivation
锥体颜料失活的机制
  • 批准号:
    7033295
  • 财政年份:
    2005
  • 资助金额:
    $ 7.57万
  • 项目类别:
Mechanisms of Cone Pigment Deactivation
锥体颜料失活的机制
  • 批准号:
    7126808
  • 财政年份:
    2005
  • 资助金额:
    $ 7.57万
  • 项目类别:
MOLECULAR ANALYSIS OF RETINAL/PINEAL MELATONIN SYNTHESIS
视网膜/松果体褪黑素合成的分子分析
  • 批准号:
    2266774
  • 财政年份:
    1990
  • 资助金额:
    $ 7.57万
  • 项目类别:
MOLECULAR ANALYSIS OF RETINAL/PINEAL MELATONIN SYNTHESIS
视网膜/松果体褪黑素合成的分子分析
  • 批准号:
    3477952
  • 财政年份:
    1990
  • 资助金额:
    $ 7.57万
  • 项目类别:
MOLECULAR ANALYSIS OF RETINAL/PINEAL MELATONIN SYNTHESIS
视网膜/松果体褪黑素合成的分子分析
  • 批准号:
    3477953
  • 财政年份:
    1990
  • 资助金额:
    $ 7.57万
  • 项目类别:
MOLECULAR ANALYSIS OF RETINAL/PINEAL MELATONIN SYNTHESIS
视网膜/松果体褪黑素合成的分子分析
  • 批准号:
    3477954
  • 财政年份:
    1990
  • 资助金额:
    $ 7.57万
  • 项目类别:
MOLECULAR ANALYSIS OF RETINAL/PINEAL MELATONIN SYNTHESIS
视网膜/松果体褪黑素合成的分子分析
  • 批准号:
    3477956
  • 财政年份:
    1990
  • 资助金额:
    $ 7.57万
  • 项目类别:

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