MUSSEL-ADHESIVE PROTEIN: A SUBSTRATE FOR CELLS IN VITRO

贻贝粘附蛋白：体外细胞的底物

• 批准号: 3487666
• 负责人: PAUL T PICCIANO
• 金额: $ 4.8万
• 依托单位: BIOPOLYMERS, INC.
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-29 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3487666
• 关键词: cell adhesion; cytotoxicity; morphology; tissue /cell culture

Within recent years, it has become recognized that many of the problems experienced in initiating cell cultures in vitro with low seeding densities or newly isolated cells are substrate-related. Specifically, studies have found that many of these problems stem from inadequate surface properties found with plastic and glass tissue culture surfaces, and lower than optimal levels in nutritive media of serum proteins specialized in promoting cell adhesion in vivi. When these molecules (fibronectin, laminin, collagen) or related proteins (gelatin, poly-d-lysine) are added to media, cell attachment and metabolism improve. Recently, a protein (mussel-adhesive protein - MAP) has been isolated from the marine mussel, Mytilus edulis, that appears to have great potential for use as an attachment factor for cells in vitro. It is biocompatible, has high tensile strength, and acts as a cement to secure M. edulis to a variety of substrates in its habitat. In addition, preliminary studies indicate that cell attachment is enhanced in the presence of MAP in vitro. As a result, the experiments within this proposal are designed as a basic study to evaluate the potential of MAP in cell adhesion. Multiple cell types will be plated on MAP and compared to conventional factors by parameters including attachment efficiency and rate, morphology and possible cytotoxicity.

近年来，人们已经认识到，许多问题 具有在低密度下启动体外细胞培养的经验 或者，新分离的细胞与底物有关。具体地说，研究表明 发现这些问题中的许多都是由于表面性质不充分造成的 发现与塑料和玻璃组织培养表面，并低于 专门化血清蛋白在营养培养基中的最佳水平 在活体内促进细胞黏附。当这些分子(纤维连接蛋白， 层粘连蛋白、胶原蛋白)或相关蛋白质(明胶、聚-D-赖氨酸)被加入 培养上清液中，细胞贴壁和新陈代谢改善。 最近，一种蛋白质(贻贝-粘附性蛋白-MAP)被分离出来。 海洋贻贝，紫贻贝，似乎有很大的潜力 在体外用作细胞的附着因子。它是生物兼容的，有 高抗拉强度，并作为粘合剂将茶树花固定在 其栖息地的底物种类繁多。此外，初步研究 表明在体外MAP存在时，细胞的黏附能力增强。 因此，本方案中的实验被设计为基本的 评价MAP在细胞黏附中的作用。多个单元格 类型将在地图上电镀，并与传统因素进行比较 参数包括附着效率和附着速率、形态和 可能有细胞毒性。